Aspectos clínicos e laboratoriais do complexo gengivite-estomatite em gatos domés / Clinic and laboratorial profile of Gingivostomatitis Complex in domestic cats

Foram incluídos 76 gatos domésticos com diferentes graus (I a IV) de lesões em cavidade oral, os quais foram avaliados clinicamente e tiveram coletadas amostras de sangue e suabes da cavidade oral. A maioria dos gatos portadores de CGE eram machos, castrados, adultos, sem raça definida e com estilo de vida confinado. Os sinais clínicos observados e associados à gravidade da inflamação na cavidade oral foram halitose (98,7%); ptialismo (22,4%); hemorragia bucal (9,2%) e úlcera na parte superior dos lábios (2,6%); desconforto à manipulação da cavidade oral (44,7%) e perda dentária (55,3%). A maioria dos gatos avaliados foi classificada no grau II (43,4%). Não se observou diferença significativa nos resultados do eritrograma dos gatos portadores de CGE, independentemente da gravidade das lesões e da sintomatologia clínica. Entretanto, observou-se neutrofilia (21,1%) e aumento de proteínas plasmáticas totais (47,3%), na maioria dos animais de grau II, sugerindo que esses parâmetros laboratoriais, quando aumentados, possam estar associados a graus menos graves de CGE. A análise das lâminas de citologia da cavidade oral dos gatos demonstrou que a presença de Simonsiella spp. foi mais frequente nos animais incluídos no grau IV, entretanto não é possível afirmar que essa bactéria esteja relacionada à gravidade das lesões.(AU)

Seventy six domestic cats classified with different scores (I to IV) of oral lesions have been included in the study, being clinically examined and having blood samples and oral cavity swabs collected. Most cats diagnosed with Gingivostomatitis Complex (GSC) were male, neutered, adult, DSH, and lived confined. Clinical signs observed and related to the severity of mouth inflammation were halitosis (98.7%), ptyalism (22.4%), oral hemorrhage (9.2%), and upper lip ulcers (2.6%); discomfort during mouth examination (44.7%) and tooth loss (55,3%). Most cats have been classified as score II (43.4%). The red blood cell count showed no significant statistical difference regardless the different scores of oral lesions and clinical symptoms. However, the leukogram showed neutrophilia (21.1%) and plasmatic proteins had an increased level (47.3%), in most of the animals classified as score II, suggesting that these parameters, when increased, may be associated to less severe GSC scores. Oral cavity microscope slides analysis showed that Simonsiella spp. have been more frequent in the cytology of the score IV animals, however, it does not allow us to correlate the presence of the bacteria with the severity lesions degree.(AU)

Seventy six domestic cats classified with different scores (I to IV) of oral lesions have been included in the study, being clinically examined and having blood samples and oral cavity swabs collected. Most cats diagnosed with Gingivostomatitis Complex (GSC) were male, neutered, adult, DSH, and lived confined. Clinical signs observed and related to the severity of mouth inflammation were halitosis (98.7%), ptyalism (22.4%), oral hemorrhage (9.2%), and upper lip ulcers (2.6%); discomfort during mouth examination (44.7%) and tooth loss (55,3%). Most cats have been classified as score II (43.4%). The red blood cell count showed no significant statistical difference regardless the different scores of oral lesions and clinical symptoms. However, the leukogram showed neutrophilia (21.1%) and plasmatic proteins had an increased level (47.3%), in most of the animals classified as score II, suggesting that these parameters, when increased, may be associated to less severe GSC scores. Oral cavity microscope slides analysis showed that Simonsiella spp. have been more frequent in the cytology of the score IV animals, however, it does not allow us to correlate the presence of the bacteria with the severity lesions degree.(AU)

Comparison of three laboratorial tests for diagnosis of canine parvovirus infection / Comparação de três testes laboratoriais para diagnóstico da infecção pelo parvovírus canino

The aim of this study was to evaluate the rapid tests currently used for canine parvovirus (CPV) diagnosis: hemagglutination test (HA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR). A total of 112 fecal samples collected from diarrheic puppies up to one year of age were tested. The EIA was able to detect CPV antigen in 44 samples. By HA, 32 samples tested highly positive with titers >128, eight tested weakly positive (titers 32 and 64) and 72 were negative (titers <16). Using PCR, 57 samples were found positive including 13 EIA-negative and 19 HA-negative samples. The best correlation was observed between EIA and PCR (88.4%). These tests were able to detect all types of CPV, including CPV-2c. Considering that 23%-33% of dogs presenting enteritis did not show infection by EIA nor HA, negative results from the antigen detection tests should be confirmed through molecular methods.

Avaliaram-se os métodos rápidos rotineiramente utilizados para diagnóstico da infecção por parvovírus canino (CPV): teste de hemaglutinação (HA), ensaio imunoenzimático (EIE) e reação em cadeia pela polimerase (PCR). Um total de 112 amostras fecais de cães diarreicos com até um ano de idade foi testado. O EIE foi capaz de detectar o antígeno do CPV em 44 amostras. Por HA, 32 amostras foram consideradas fortemente positivas com títulos >128, oito fracamente positivas (títulos 32 e 64) e 72 negativas (títulos <16). Por PCR, 57 amostras foram positivas incluindo 13 EIE-negativas e 19 HA-negativas. A melhor correlação foi observada entre EIE e PCR (88,4%). Os testes foram capazes de detectar todos os tipos de CPV, incluindo o CPV-2c. Considerando-se que em 23%-33% dos filhotes com enterite a infecção por CPV não foi diagnosticada pelos testes de EIE e HA, os resultados negativos nos testes de detecção de antígeno devem ser confirmados por meio de métodos moleculares.