Antimicrobial screening of Garlic (Allium sativum) extracts and their effect on Glucoamylase activity in-vitro.

Amongst the various extracts, Petrol-ether and Chloroform extracts of garlic were found to be good antimicrobial agents against Escherechia coli, Pseudomonas Aeruginosa, Bacilli Subtilis and Staphylococcus Aureus, and moderate inhibitors of Glucoamylase, an enzyme prominent in carbohydrate metabolism. Whereas, the Ethyl acetate and Methanolic extracts did not exhibit antimicrobial activity and found moderate to good activators of Glucoamylase.

Effect of Epigallocatechin gallate isolated from Terminalia Bellerica fruit rind on glucoamylase activity in vitro.

Chloroform-Ethyl Acetate fraction of Terminalia bellerica fruit rind powder showed maximum antimicrobial activity. This fraction on further purification by column chromatography gave a single spot compound, which after characterisation was found to be Epigallocatechin gallate. Epigallo catechin gallate showed significant antimicrobial activity against E.coli, B. subtilis and S. Aureus. Epigallocatechin gallate inhibits 23.13 to 46.24 % activity of glucoamylase at very low concentrations, therefore may be used as hypoglycaemic agent showing good agreement with the earlier literature.