Effects of different types of dentin protective membranes on dentin adhesion properties / 西安交通大学学报(医学版)

Objective: To investigate the effects of three different types of dentin protective membranes (HyC, SBU and S3) on dentin adhesive properties. Methods: Fresh isolated molar teeth were collected, and smooth dentin surface was obtained after embedding, cutting and polishing. In the control group, dentin protective membrane was not applied, while the remaining three groups were coated with three different types of dentin protective membranes, and the preformed resin prosthesis was cemented with resin cement. A strip dentin-resin sample with a cross-sectional area of 0.9 mm×0.9 mm was obtained by a precision cutting machine. The tensile force was tested at the loading speed of 0.5 mm/min with a universal testing machine until the dentin-resin strip was broken. The maximum tensile force during the loading process was automatically recorded by the computer, and the microstructure and fracture mode of the section were observed by a scanning electron microscope. Results: After coating dentin protective membranes, the tensile strength of each experimental group was significantly increased compared with that of the control group (P<0.05), and the tensile strength of group HyC was significantly smaller than that of group SBU and group S3 (P<0.05). The tensile fracture mode shown by microstructure observation was mainly interfacial failure. Conclusion: Different types of dentin protective membranes can increase the adhesive properties between dentin and resin prosthesis. SBU and S3 can improve the adhesion strength more significantly than HyC.

Effect of Different Hemapheresis Procedures on Components of Hematopoietic Stem Cells Collected from Healthy Donors / 中国实验血液学杂志

<p><b>OBJECTIVE</b>To investigate the effects of different hemapheresis procedures on the components of hematopoietic stem cells(HSCs) collected from helathy donors.</p><p><b>METHODS</b>twelve donors were underwent stem cell collection from January 2015 to August 2016, and the stem cells were randomly colleted by AutoPBSC procedure of COBE spectra and MNC procedure of the Spectra Optia blood cell separator, the mononuclear cells, CD34cells, granulocytes, lymphocytes and platelets in the collections were compared.</p><p><b>RESULTS</b>The circulating blood volume, the acquisition time and dosage of anticoagulants were not significantly different between two procedures. The volume and the mononuclear cell count collected by AutoPBSC procedure were lower than those by the MNC procedure, while the CD34cell count by AutoPBSC procedure was higher than that by the MNC procedure. More lymphocytes and platelets were collected by AutoPBSC procedure as compared with that by the MNC procedure (P<0.05).</p><p><b>CONCLUSION</b>Compared with MNC procedure of the Spectra Optia blood cell separator, the number of collected stem cells, lymphocytes and platelets are higher by using AutoPBSC procedure of the COBE spectra blood cell separator.</p>