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Objective:To observe the changes in bone mineral density and microstructure parameters in sclerostin (SOST) gene knockout (SOST -/-) mice after ovariectomy. Methods:Twelve 4-week-old SOST knockout mice were randomly divided into two groups ( n=6): ovariectomized group (SOV), sham operated group (SSO). Twelve wild-type mice were randomly divided into two groups ( n=6): wild-type ovariectomized group (WTO), wild-type sham operated group (WTS). Twelve weeks later, mice were sacrificed and one lumbar vertebra of each mouse was selected for micro-CT analysis. The bone mineral density, trabecular volume fraction, trabecular number and trabecular thickness were observed and compared in the 4 groups. Results:There was no difference in bone mineral density, trabecular volume fraction, trabecular number and trabecular thickness between SOV and SSO groups. Bone mineral density, trabecular volume fraction, trabecular number and trabecular thickness in SOV and SSO groups were significantly higher than those in WTO and WTS groups ( P<0.001). Bone mineral density, trabecular volume fraction and trabecular number in WTO group were significantly lower than those in WTS group ( P=0.017, 0.039, 0.021, respectively). There was no difference in trabecular thickness between WTO and WTS groups. Conclusions:Sclerostin knockout mice showed high bone mass, and ovariectomy did not lead to bone loss and bone microstructure degeneration, which indicates that slerostin is a potential therapeutic target for postmenopausal osteoporosis.
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Objective To observe the changes in bone mineral density and microstructure parameters in sclerostin (SOST) gene knockout mice treated with glucocorticoid.Methods 12 4-week-old SOST knockout mice were randomly divided into two groups (n =6):methylprednisolone intervention group [SOM group,methylprednisolone 3 mg/(kg· d),subcutaneous injection],placebo group (SOS group,isovolumetric saline subcutaneous injection).12 wild-type mice were randomly divided into two groups (n =6):wild-type placebo group (WTS group,isovolumetric saline subcutaneous injection),wild methylprednisolone intervention group [WTM group,methylprednisolone 3 mg/(kg · d),subcutaneous injection].12 weeks later,mice were sacrificed and one lumbar vertebra of each mouse was selected for microCT analysis.Results There was no difference in bone mineral density (BMD),trabecular volume fraction,trabecular number and trabecular thickness between SOM and SOS groups (P ＞ 0.05).BMD,trabecular volume fraction,trabecular number and trabecular thickness in SOM and SOS groups were significantly higher than those in WTS and WTM groups (P ＜0.05).BMD,trabecular volume fraction,trabecular number and trabecular thickness in WTM group were significantly lower than those in WTS group (P ＜ 0.05).Conclusions Sclerotin gene knockout mice can resist glucocorticoid-induced bone loss and bone microarchitectural deterioeration.The treatment of osteoporosis with SOST/sclerotin as a target will be an effective method in the future.
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Objective@#To observe the changes in bone mineral density and microstructure parameters in sclerostin (SOST) gene knockout mice treated with glucocorticoid.@*Methods@#12 4-week-old SOST knockout mice were randomly divided into two groups (n=6): methylprednisolone intervention group [SOM group, methylprednisolone 3 mg/(kg·d), subcutaneous injection], placebo group (SOS group, isovolumetric saline subcutaneous injection). 12 wild-type mice were randomly divided into two groups (n=6): wild-type placebo group (WTS group, isovolumetric saline subcutaneous injection), wild methylprednisolone intervention group [WTM group, methylprednisolone 3 mg/(kg·d), subcutaneous injection]. 12 weeks later, mice were sacrificed and one lumbar vertebra of each mouse was selected for micro-CT analysis.@*Results@#There was no difference in bone mineral density (BMD), trabecular volume fraction, trabecular number and trabecular thickness between SOM and SOS groups (P>0.05). BMD, trabecular volume fraction, trabecular number and trabecular thickness in SOM and SOS groups were significantly higher than those in WTS and WTM groups (P<0.05). BMD, trabecular volume fraction, trabecular number and trabecular thickness in WTM group were significantly lower than those in WTS group (P<0.05).@*Conclusions@#Sclerotin gene knockout mice can resist glucocorticoid-induced bone loss and bone microarchitectural deterioeration. The treatment of osteoporosis with SOST/sclerotin as a target will be an effective method in the future.
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Objective To explore the risk factors of gestational diabetes mellitus and its influence on pregnancy outcome,and to provide guidance for early intervention of gestational diabetes to improve pregnancy outcome in clinic.Methods Totally 159 patients with gestational diabetes mellitus in our hospital from January 2014 to January 2016 as observation group were collected,and at the same time,634 healthy pregnancy women as control group were collected.The risk factors of gestational diabetes mellitus were analyzed by the Logistic regression analysis and comparison of pregnancy outcome between two groups was conducted.Results The patients in the observation group with age ≥ 35 years,overweight or obesity before pregnancy,adverse pregnancy history,family history of diabetes,hypertension and vaginal candida and thyroid peroxidase antibody (TPOAb) compared with the control group,the difference was statistically significant (P ＜ 0.05).Multivariate logistic regression analysis showed that age ≥ 35 years,overweight or obesity before pregnancy,adverse pregnancy history,family history of diabetes,and positive TPOAb were the independent risk factors of gestational diabetes mellitus,OR was 2.441,2.889,1.486,4.879,and 1.323,respectively;the difference was statistically significant (P ＜ 0.05).The incidence of premature rupture of membrane,pregnancy hypertensive disorder,premature birth,polyhydramnios,uterine-incision and postpartum hemorrhage in observation group were higher than those in control group,the difference was statistically significant (P ＜ 0.05).The incidence of giant baby,fetal distress and mild neonatal asphyxia were higher than those in control group,and the difference was statistically significant (P ＜ 0.05).No statistical significance in fetal growth restriction,neonatal malformations between two groups was found (P ＞ 0.05).Conclusions Age ≥35 years,overweight or obesity before pregnancy,adverse pregnancy history,family history of diabetes,and thyroid dysfunction were the independent risk factors of gestational diabetes mellitus,and it has serious effect on pregnancy outcome,so that it should take measures to prevent the independent risk factors.
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Objective To analyze the correlation between different kidney function damage caused by type 2 diabetes and the occurrence of osteoporosis.Methods Collect the clinical data of 112 patients of type 2 diabetes during the Sep.2008 to Mar.2011,and proceed statistical examination of kidney function and bone mineral density from different location.Analyze the correlation between different kidney function damage caused by type 2 diabetes and the occurrence of osteoporosis by using statistical software.Results Among all different kidney function damage caused by type 2 diabetes,the order of severity of occurrence of osteoporosis was increasing in pace with the more severe damaging kidney function.There was a significant difference between the two samples(33.3％,44.8％,56.5％,80.0％,P ＜ 0.05).Conclusion The correlation between different kidney function damage caused by type 2 diabetes was positively correlated with the occurrence of osteoporosis.We should strengthen to control and cure the patient's kidney function damage to decrease the occurrence of osteoporosis.