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Objective:To observe the effects of static 70° head-up tilted standing and of repeated body repositioning on hemodynamics in healthy young and middle-aged persons.Methods:The hemodynamics of 24 middle-aged and 23 younger persons were studied. Both groups were requested to perform static 70° head-up tilted standing and to repeatedly change their body position from 0° to 70° of tilt at a velocity of 1°/second for ten minutes in a random order. Before, between and after each test the subjects rested supine for ten minutes. Hemodynamic variables and blood pressure were recorded non-invasively.Results:The average heart rate (HR) increased significantly in both groups when rising from supine to the testing positions. In 70° tilted standing the average HR of the youth group, 84.0±9.5bpm, was significantly higher than that in the other position and that of the middle-aged group in the same position. The average HR of the middle-aged group in 70° tilted standing was also significantly higher than in the other position. Among the middle-aged group, the average stroke volume (SV) in the testing positions was significantly lower than when resting. Significant differences were observed in the average diastolic blood pressure (DBP) between the testing and rest positions for both groups, with the average DBP of the middle-aged group significantly higher than that of the youth group in all three positions. Among the youth group, the average SV, CO and systolic blood pressure (SBP) of the males were significantly higher than among the females in all of the different body positions.Conclusions:Young persons mainly rely on an increased heart rate to maintain cardiac output while middle-aged participants appear to achieve this through increased peripheral resistance. Repeated position changes have less impact on hemodynamics than 70° inclined standing, making it a safer and more stable training method. However, the long-term effects of such intervention need to be confirmed in further studies.
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Objective To establish a molecular typing system of Staphylococcus aureus by using resolution melting for food-poi-soning fast tracing.Methods Primers were designed and synthesized according to the literature of VNTR in Staphylococcus au-reus ,and were used to perform molecular typing on the strains which had detected by PFGE,then 4 types of VNTRs were with higher discriminatory power were selected.On this basis,we established a molecular typing system for the detection of 59 Staphy-lococcus aureus isolated from food poisoning.Results The molecular typing system has good precision for detection.The standard deviation(s)of within-batch repetitive experiments were 0.03 -0.05 ℃,between-batch repetitive experiments were 0.04 -0.06℃,between-day repetitive experiments were 0.04-0.06 ℃.At the same time,the 59 strains of Staphylococcus aureus were divided into 19 types which were 11 epidemic clones and 8 sporadic clones.The correlation coefficient of Simpson was 0.916 4.Conclusion The molecular typing system for Staphylococcus aureus based on resolution melting was simple,fast and repeatable.It can be ap-plied to fast tracing and screen of Staphylococcus aureus in food poisoning.
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OBJECTIVE To observe the clinical efficacy of two kinds of compound skin disinfectants(compound iodine and Anerdian).METHODS One hundred and twenty recruited patients were divided into three groups randomly,group A(compound iodine group),group B(Anerdian group) and control group(iodine tincture group),40 patients per group.The groups A and B were carried on with one-step skin greasing disinfection.The control group was carried on with routine two-steps skin greasing disinfection.The sampling and detection were carried on by The Technological Specification of Hospital Disinfection published by the Ministry of Health.RESULTS The kill ratio of three groups was(95.44?9.45)%,(95.29?7.21)% and(96.24?11.12)%,respectively.Their disinfection qualification ratio was 97.50%,95.00% and 97.50%,respectively.Comparing the kill ratio and disinfection qualification ratio,there was no significant difference among three groups.CONCLUSIONS The disinfection efficacy of two kinds of the compound skin disinfectants are definite,there is no difference comparing with the traditional iodine tincture and ethanol disinfectants,but they are easy to carry on,safe and practical and the time is shorter,saving the time and the labor.It is helpful to increase the work efficiency of the nurse and deserve to be extended in the clinical application.