Network pharmacological study of Schizonepetae Herba and Saposhnikoviae Radix in treatment of ulcerative colitis / 中国中药杂志

The aim of this paper was to screen the active targets of Schizonepetae Herba and Saposhnikoviae Radix in the treatment of ulcerative colitis by means of network pharmacology,and to investigate their mechanism of action. The effective components of Schizonepetae Herba and Saposhnikoviae Radix were screened out by traditional Chinese medicine systematic pharmacological( TCMSP)database,with oral bioavilability( OB) ≥30% and drug-like( DL) ≥18% selected as the thresholds. Target PPI network was built between the main components and their corresponding targets. One hundred and eighty-two human genes corresponding to the medicine target sites were obtained from Uniprot database; 3 874 genes corresponding to ulcerative colitis were obtained from Genecard database.A total of 115 intersection genes were screened from disease genes and medicine genes,and the PPI interaction analysis was conducted by using String tool. Disease-target PPI network was drawn by using Cytoscape software,and component-target-disease network was constructed. One hundred and eight nodes and 1 882 connections were found,and then Cytoscape software was used to merge the networks and filter the core network for gene GO function analysis and KEGG pathway enrichment analysis. The mechanism of Schizonepetae Herba and Saposhnikoviae Radix was then verified by animal experiment. Gene GO functional analysis suggested that biological process,molecular functions and cell components were involved,and it was found that ulcerative colitis might be related to transcription factor activity,and cytokine receptor binding,etc. Gene KEGG pathway enrichment analysis showed that the mechanism of ulcerative colitis might be associated with TNF and Toll-like receptors( TLRs) signaling pathway-mediated cytoinflammatory factors interleukin-1( IL-1) and interleukin-6( IL6). The possible mechanism of the effective components of Schizonepetae Herba and Saposhnikoviae Radix in treating ulcerative colitis might be related to intervening the cytokine receptor binding of TNF and TLRs signaling pathways,reducing the transcription of nuclear factor-kappaB( NF-κB),and inhibiting the secretion of intestinal inflammatory factors IL-1 and IL-6.

Study on recent sedentary behaviors and influencing factors among children and adolescents in Chongqing area / 上海交通大学学报（医学版）

Objective: To describe the current status and explore the associated factors of sedentary behaviors among children and adolescents residing in Chongqing area. Methods: By random stratified and cluster sampling, a total of 9 596 students aged from 6 to 18 years old in Chongqing urban districts together with their parents were enrolled through a questionnaire. Mann-Whitney U test and Kruskal-Wallis H test were used to compare the difference among groups, and the binary Logistic regression model was used to explore the factors that influenced the prevalence of average time of homework>2 h/d and screen time>2 h/d. Results: The average time of sedentary behaviors in school days was 2.82 h/d, and the rate of sedentary behaviors>2 h/d was 74.1%. The rate of boys (75.3%) was significantly higher than that of girls (72.7%) (χ2=8.333, P=0.000). Logistic regression analysis showed that junior and senior students were the common risk factors for both homework and screen time>2 h/d; on the contrary, extracurricular sports never being occupied could be the common protective factors. In addition, being girls (OR=1.187, 95% CI 1.048-1.299) and parents' sedentary time>2 h/d (OR=1.529, 95% CI 1.303-1.794) could be the risk factors for homework time>2 h/d. Non-only child (OR=1.194, 95% CI 1.066-1.320), no family rules on screen time (OR=1.193, 95% CI 1.062-1.388), and having a TV set at home (OR=1.330, 95% CI 1.147-1.472) could be the risk factors for screen time>2 h/d. Conclusion: The status quo of sedentary behaviors among students in Chongqing area is not quite well. School's policy on extracurricular sports and home screen environment may be the mainly modifiable influencing factors.

Study on recent sedentary behaviors and influencing factors among children and adolescents in Chongqing area / 上海交通大学学报（医学版）

Objective·To describe the current status and explore the associated factors of sedentary behaviors among children and adolescents residing in Chongqing area.Methods·By random stratified and cluster sampling,a total of 9 596 students aged from 6 to 18 years old in Chongqing urban districts together with their parents were enrolled through a questionnaire.Mann-Whitney U test and Kruskal-Wallis H test were used to compare the difference among groups,and the binary Logistic regression model was used to explore the factors that influenced the prevalence of average time of homework＞2 h/d and screen time＞2 h/d.Results·The average time of sedentary behaviors in school days was 2.82 h/d,and the rate of sedentary behaviors＞2 h/d was 74.1％.The rate of boys (75.3％) was significantly higher than that of girls (72.7％) (x2=8.333,P=0.000).Logistic regression analysis showed that junior and senior students were the common risk factors for both homework and screen time＞2 h/d;on the contrary,extracurricular sports never being occupied could be the common protective factors.In addition,being girls (OR=1.187,95％ CI 1.048-1.299) and parents' sedentary time＞2 h/d (OR=1.529,95％ CI 1.303-1.794) could be the risk factors for homework time＞2 h/d.Non-only child (OR=1.194,95％ CI 1.066-1.320),no family rules on screen time (OR=1.193,95％ CI 1.062-1.388),and having a TV set at home (OR=1.330,95％ CI 1.147-1.472) could be the risk factors for screen time＞2 h/d.Conclusion·The status quo of sedentary behaviors among students in Chongqing area is not quite well.School's policy on extracurricular sports and home screen environment may be the mainly modifiable influencing factors.

Dynamic Characteristics of Serum Hepatitis B Surface Antigen in Chinese Chronic Hepatitis B Patients Receiving 7 Years of Entecavir Therapy / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>The ultimate goal of hepatitis B treatment is hepatitis B surface antigen (HBsAg) seroclearance. Several factors have been suggested to be associated with the rate of HBsAg reduction in antiviral-naive or lamivudine therapy cohorts. However, there are few studies evaluating the factors during long-term entecavir (ETV) therapy. In the present study, we aimed to evaluate the factors to predict the outcome of ETV therapy for 7 years.</p><p><b>METHODS</b>A total of 47 chronic hepatitis B (CHB) patients treated with ETV monotherapy were included in this study. Liver biochemistry, hepatitis B virus (HBV) serological markers, serum HBV DNA, and HBsAg titers were tested at baseline, 3 months, 6 months, and yearly from 1 to 7. The associations between factors and HBsAg reduction were assessed using multivariate tests with repeated measure analysis of variance.</p><p><b>RESULTS</b>At baseline, serum HBsAg levels showed a positive correlation with baseline HBV DNA levels (r = 0.625, P < 0.001). The mean HBsAg titers after ETV treatment were significantly lower than the baseline titers (P ranges from 0.025 to 0.000,000,6). The HBsAg reduction rate during the 1st year was greater compared to after 1 year of treatment (P < 0.05). Multivariate test showed that hepatitis B e antigen (HBeAg) seroclearance and/or HBsAg reduction ≥0.5 log10 IU/ml at 6 months had a high negative predictive value (96.77%) for HBsAg seroclearance (P = 0.002, P = 0.012, respectively).</p><p><b>CONCLUSIONS</b>The HBsAg reduction rate during the 1st year was greater than that after 1 year of treatment. Further, HBeAg status and HBsAg levels at month 6 are the optimal factors for the early prediction of HBsAg seroclearance after long-term ETV therapy in CHB patients.</p>

Antiviral nucleotide-induced dynamic change of HBV DNA and HBsAg and significance of quarterly and annual quantitative measurements over 5-year follow-up of chronic hepatitis B patients / 中华肝脏病杂志

<p><b>OBJECTIVE</b>To analyze the dynamic changes in hepatitis B virus (HBV) DNA and hepatitis B surface antigen (HBsAg) levels in chronic hepatitis B (CHB) patients following treatment by antiviral nucleotide drugs over a 5-year follow-up period and to assess the clinical significance of quarterly and annual quantitative measurements.</p><p><b>METHODS</b>One-hundred-and-ten patients with CHB were enrolled in the study and administered on-going standard mono-therapy with various antiviral nucleotide drugs. Over a 5-year period, the HBV DNA level was measured by quantitative PCR every three months and the HBsAg levels were measured by chemiluminescence once a year. The dynamic changes in HBV DNA and HBsAg levels were assessed by Chi-squared test and ANOVA.</p><p><b>RESULTS</b>Only 90 of the CHB patients completed the 5-year follow-up and were included in the analysis. The patients who showed HBeAg-positivity at baseline (study start) had higher levels of HBV DNA and HBsAg than the patients showing HBeAg-negativity. In general, the antiviral nucleotide drug therapy induced downward trends in HBsAg and HBV DNA level over time (F = 17.1, 151.53, all P less than 0.05). However, the most robust reduction in HBV DNA occurred during the first year. The HBsAg level followed an opposite trend, with the most robust reductions occurring in the 3rd, 4th and 5th years of treatment.</p><p><b>CONCLUSION</b>Long-term antiviral nucleotide mono-therapies induced decreases in HBV DNA and HBsAg levels in CHB patients, with the former being most reduced in the short-term and the latter in the long-term.</p>

Construction of quantitative real-time PCR detection system of transgenic tomato line Zeneca B,Da,F / 南方医科大学学报

<p><b>OBJECTIVE</b>To construct the plasmid reference molecules for detection of transgenic tomato line Zeneca B,Da,F using quantitative real-time PCR(qPCR).</p><p><b>METHODS</b>Three plasmid reference molecules pEasy-T3-APX, pEasy-T3-16A and pEasy-T3-16S were cloned based on reverse genetics, which contain the target fragments of tomato endogenous reference gene apx (ERG-apx), gene-specific sequence of pg(GS-pg) and construct-specific sequence of vectors pJR16S/pJR16A (CS-16S/CS-16A) of Zeneca B,Da,F, respectively. Primers and Taqman probes were designed by Beacon Designer 7.5.The specificity, sensitivity, reproducibility and the limit of detection(LOD) of the qualitative and quantitative PCR system based on the plasmid reference molecules were evaluated. PicoGreen was used to measure the DNA concentration of the plasmid reference molecules. Two sets of samples containing 1% or 0.1% (w/w) pEasy-T3-16A or pEasy-T3-16S mixed with pEasy-T3- APX as background DNA were prepared for evaluating the efficacy of the qPCR system.</p><p><b>RESULTS</b>The target fragments for qPCR detection were anchored, ERG-apx 108 bp, GS-pg 108 bp , CS-16S 109 bp and CS-16A 102 bp. The three plasmid reference molecules were confirmed at the expected sizes by restriction enzyme digestion. The qPCR results showed that the RSD of reproducibility were 0.2% to1.5%, LOD was 25 copies, R2 values for these standard curves were 0.994 ~0.998 and amplification efficiencies were 93.3%~102.4%.The bias between the test and true values of two sets of mixed samples ranged from -9.3% to 14.7% after adjusting by conversion factors(Cf).</p><p><b>CONCLUSION</b>The plasmid reference molecules and qPCR system for qualitative and quantitative detection of transgenic tomato line Zeneca B,Da,F have been established successfully.</p>

Treatment for Sialorrhea in Patients with Amyortophic Lateral Sclerosis (review) / 中国康复理论与实践

@#Amyotrophic lateral sclerosis(ALS)is a rare progressive motor neuron disease.Sialorrhoea is one of the common symptoms which influenced the patient's quality of life.This paper reviewed the treatments for sialorrhoea in ALS patients.

Nursing Care for Patients Accepting Pulse Steroid Therapy / 中国康复理论与实践

@#Objective To establish the nursing care for the patients with pulse steroid therapy, and to investigate its effect. Methods 78 patients who accepted pulse steroid therapy in the past 4 years were analyzed retrospectively, in which, 42 within past 2 years （2008 and 2007) were as pulse steroid nursing care group, and other 36 patients before 2 years (2007 and 2006) were as routine nursing care group. Results 3 weeks after the pulse steroid therapy, in pulse steroid nursing care group, proximal muscle weakness were found in 8 patents, insomnia in 18 patients; while in routine nursing care groups, proximal muscle weakness were found in 16 patents, and insomnia in 25 patients（P<0.05）. Conclusion Pulse steroid nursing care can reduce the incidence of muscle weakness and insomnia after pulse steroid therapy significantly.

Neuralgic Amyotrophy:8 Cases Report and Relevant Literatures Review / 中国康复理论与实践

@# Objective To investigate the clinical features and characteristic of auxiliary examination of neuralgic amyotrophy(NA).Methods8 patients with idiopathic neuralgic amyotrophy were analyzed retrospectively with literature review.Results and ConclusionAntecedent events were found before attack in 5 patients.Serious pain,which was followed by paresis and atrophy,as initial symptom,was found in 7 patients.Damage in the upper and middle trunk distribution occurred most frequently.Elevated liver enzymes were found during the early phase of their attacks in 2 patients.CSF analysis was performed in 5 patients,and mildly elevated protein was found in two.5 patients had been misdiagnosed as cervical spondylosis.After medical and/or physical therapy(6 patients with corticosteroid and physical therapy,2 only with physical therapy),all the patients had been followed up for 2～13 months.Muscle power was recovered in 67% of all the patients,partially recovered in 33%.

Effects of Ziwuliuzhu combined selection of the source point and the collateral point on athletic injuries and the state of channels / 中国针灸

<p><b>OBJECTIVE</b>To probe into therapeutic effects of Ziwuliuzhu combined selection of the source point and the collateral point on athletic injuries and the state of channels.</p><p><b>METHODS</b>Ninety cases meeting diagnostic criteria were randomly divided into a group of Ziwuliuzhu combined selection of the source point and the collateral point (group A), a group of routine selection of acupoint (group B) and a group of external application of medicine (group C), 30 cases in each group. The electric conduction amount on source points of 12 channels were determined before and after treatment with a point diagnosis and treatment instrument. Changes of the state of channels and clinical therapeutic effects in the 3 groups were investigated after treatment.</p><p><b>RESULTS</b>The therapeutic effect in the group A was better than those in the other two groups (P < 0.05).</p><p><b>CONCLUSION</b>Ziwuliuzhu combined selection of the source point and the collateral point has good effect on pain and other clinical symptoms of athletic injuries, and makes channels of imbalance tend to balance or recover balance.</p>