The Effect of Anabolic Steroid on Body Composition and Protein Metabolism in Malnourished CAPD Patients / 대한신장학회잡지

To evaluate the effect of anabolic steroid(AS) on body composition and protein metabolism in malnourished CAPD patients, 7 CAPD patients who were proved to be malnourished by subjective global assessment received 200mg of AS, nandrolone decanoate, by intramuscular route monthly for 5 months. We analyzed the body composition, biochemical nutritional markers, plasma and dialysate amino acids(AA) before and after administration of AS. The mean age was 53.6+/-11.5 years, the sex ratio was 2:5, and the duration of CAPD was 28.5+/-18.7 months. Two patients were diabetics. Five months after AS administration, %lean body mass seemed to increase from 52.6+/-11.7 to 57.3+/-11.0%(P>0.05), but there were no significant changes in body weight and body mass index. Serum insulin-like growth factor-I (172.4+/-88.5 vs. 204.1+/-85.8ng/ml, P<0.05) and transferrin(213.6+/-55.4 vs. 239.0+/-55.3mg/dl, P<0.05) were significantly increased, and serum total CO2(31.1+/-4.5 vs. 25.9+/-2.5mM/L, P<0.05) were significantly decreased. Urea nitrogen appearance(4.0+/-2.0 vs. 3.6+/-1.6g/ day) seemed to be decreased. Both plasma essential AA(EAA) level(738.3+/-206.5 vs. 492.5+/-187.2micromol/L, P<0.05) and peritoneal EAA loss(565.0+/-186.1 vs. 377.4+/-113.9micromol/day, P<0.05) decreased significantly after administration. Plasma total and non- essential amino acids(NEAA) level tended to be increased and peritoneal total and NEAA loss seemed to be decreased without statistical significance. In conclusion, AS seems to have anabolic effect and be useful in the correction of malnutrition in CAPD patients.

Regulatory Effect of Alveolar Macrophage Released Factor on Pulmonary Fibrosis in Vitro / 대한산업의학회지

Interstitial lung disorders are characterized by chronic inflammation of the lower respiratory tract that include increased numbers of activated alveolar macrophages and fibroblasts. These increased numbers of fibroblasts may be influenced by the alveolar macrophage released factor which was known as alveolar macrophage derived growth factor. To evaluate this hypothesis, alveolar macrophages from bronchoalveolar lavage fluid in rat were incubated with various stimulants including lipopolysaccharide (LPS), free silica dust(Si02), natural carbon dust(NC) for 4 hours and we added these supernatants to the culture of fibroblasts. And we evaluated the fibroblast proliferation, ATP and protein in 1, 2, 3 days. The results were as follows; 1. The number of fibroblasts: in groups of LPS and Si02 show significant increase in comparison with the control group but there was no; difference between NC and control groups. 2. The measurements of ATP in groups of LPS and Si02 tended to be higher than those in the control, and also higher in NC group at the 2nd day than those of control. 3. The amount of protein in LPS and Si02 increased markedly compared with the control group but there was no difference between NC and control groups. 4. In LPS group, we can observe the decrease of ATP and protein after the peak at the 1st day, but;Si02 group show the continuous increase of ATP and protein during the observation period. 5. Increased proportion of ATP and protein indicated their sensitive changes compared with the fibroblast proliferation. These results suggest alveolar macrophages act as the important integrator of the fibrotic process in interstitial lung disorders.