RESUMO
BACKGROUND:Stem cells,characterized with convenient collection,wide sources and great potential,have been developed in the cell therapy.The most commonly used stem cells include bone marrow mesenchymal stem cells,placental mesenchymal stem cells and umbilical cord blood mesenchymal stem cells.OBJECTIVE:To compare the biological changes in bone marrow mesenchymal stem cells,placental mesenchymal stem cells and umbilical cord blood mesenchymal stem cells after co-cultured with nerve cells.METHODS:Human bone marrow mesenchymal stem cells,human placental mesenchymal stem cells and human umbilical cord blood mesenchymal stem cells were isolated and cultured in vitro.The morphological changes of mesenchymal stem cells differentiated into nerve cells were observed by co-culture with Transwell culture plates.The expression of neuron-specific enolase was detected after induction.RESULTS AND CONCLUSION:In the co-culture system,these three kinds of mesenchymal stem cells gradually formed a star-like shape and were interconnected.Moreover,all the cells were positive for neuron-specific enolase.These findings reveal that the microenvironment provided by nerve cells can induce and promote the differentiation of three kinds of mesenchymal stem cells into neuron-like cells.