Effect of intensive blood glucose control on quality of life in elderly patients with type 2 diabetes in Anhui Province / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Intensive blood glucose control is proven to be associated with the diabetic microvascular and macrovascular complications, which could affect quality of life (QOL). This study was performed to determine the effects of intensive glucose control therapy on QOL of elderly patients with type 2 diabetes in Anhui Province.</p><p><b>METHODS</b>Ninety-seven elderly patients with type 2 diabetes in Anhui were randomly assigned to standard treatment group and intensive therapy group. All patients were followed up for five years on average. Correlated information has been collected during the regular follow-up.</p><p><b>RESULTS</b>Patients with microvascular complications reported significantly lower European Quality of Life-5 Dimensions (EQ-5D) scores and had more problems with usual activities, pain and anxiety than those without complications (P < 0.05). Patients having experienced hypoglycemic episodes had significantly more problems with anxiety than those without hypoglycemic episodes (P < 0.05). No significant difference was detected in all dimensions in quality of life, as well as in Visual Analog Scale score between two groups (P > 0.05). There was no significant difference in quality of life at the fifth year compared with that of the first year in both groups. Women had more feelings of pain and anxiety than men (P < 0.05) and longer disease course was associated with increased levels of pain and anxiety (P < 0.05), as well as with lower QOL. In addition, patients with higher body mass index (BMI) had more problems with daily activities than patients with lower BMI (P < 0.05).</p><p><b>CONCLUSIONS</b>Anxiety is common in elderly diabetic patients and they experienced frequent hypoglycemic episodes. Diabetic vascular complications significantly affect QOL of the patients. Intensive glucose control has no significant effect on QOL of the diabetic patients. Female, older age, long disease course, less education and high BMI are all factors caused reduced QOL and patients with these factors should be given more psychological support. Frequent mild hypoglycemic episodes do not cause impaired function of the central nervous system.</p>

Study on association of PPP1R3 gene 5 bp deletion/insertion within 3'-untranslated region polymorphism with type 2 diabetes / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To determine whether the muscle-specific glycogen-targeting regulatory subunit of the glucogen bound protein phosphatase 1 (PPP1R3) gene 5 bp deletion/insertion(D/I) within 3'-untranslated region ( 3'-UTR) polymorphism is associated with type 2 diabetes in Chinese Han population in Hefei region of Anhui province.</p><p><b>METHODS</b>The PPP1R3 gene 3'-UTR 5 bp D/I polymorphism was detected by polymerase chain reaction in 268 patients with type 2 diabetes and 106 normal controls.</p><p><b>RESULTS</b>(1) The distributions of the frequency of three genotypes and two alleles of the PPP1R3 gene 5 bp D/I polymorphism showed no significant difference between the type 2 diabetic cases and the normal controls. (2) In both the cases and controls, there was no significant difference in age at onset, duration of disease, blood glucose, blood lipid profile, blood pressure, insulin sensitive index, body mass index, and waist hip ratio between the three genotypic groups(P 0.05). (3) The PPP1R3 gene 3'-UTR polymorphism in Chinese Han population in Hefei region of Anhui province was found to be similar to that in both Japanese population and Canadian population, and to be different from that in Piman Indians and the Caucasians in Sweden.</p><p><b>CONCLUSION</b>The PPP1R3 gene 5 bp D/I within 3'-UTR polymorphism taking on genetic variation among the different races of mankind may not play a critical role in the development of type 2 diabetes mellitus in Chinese Hans of Hefei region in Anhui province.</p>

Study on the association of PPP1R3 gene polymorphism with type 2 diabetes in Han population of Anhui province / 中华流行病学杂志

<p><b>OBJECTIVE</b>To study the association of muscle-specific glycogen-targeting regulatory subunit of the glucogen-bound protein phosphatase 1 (PPP1R3) gene codon 905 Asp/Tyr polymorphism with type 2 diabetes in Chinese Han population in Hefei region of Anhui province.</p><p><b>METHODS</b>PPP1R3 gene Asp905Tyr polymorphism was detected by polymerase chain reaction and appropriate restriction enzyme (PCR-RFLP) in 262 type 2 diabetic cases and 104 normal controls. Case and control groups were divided into subgroups by body mass index (BMI) 25 kg/m2.</p><p><b>RESULTS</b>When PPP1R3 gene Asp905Tyr polymorphism was not associated with type 2 diabetes mellitus. When subjects with BMI < 25 kg/m2 and Tyr/Tyr genotypes were used as reference. Subjects with Asp905 and BMI > or = 25 kg/m2 had a 3.69-fold increase of risk suffering from type 2 diabetes (OR = 3.69, 95% CI: 1.38-8.89, P=0.006).</p><p><b>CONCLUSIONS</b>PPP1R3 gene Asp905Tyr polymorphism did not seem to play a critical role in the development of type 2 diabetes mellitus in Han population of Chinese in Anhui province but interaction between the Asp905 and BMI cause the increase of risk of type 2 diabetes.</p>

Effects of pioglitazone and tumor necrosis factor-? on adiponectin receptor mRNA expression in 3T3-L1 adipocytes / 中华内分泌代谢杂志

The effects of pioglitazone (PIO) and tumor necrosis faetor-?(TNF-?) on two kinds of adiponectin receptor (AdipoR) mRNA expression were observed in 3T3-L1 adipocytes by RT-PCR.AdipoR mRNA expression was up-regulated during 3T3-L1 preadipocyte differentiation;PIO could increase the AdipoR mRNA level expressed in undifferentiated and differentiated 3T3-L1 adipocytes in a time-and dose-dependent manner,and TNF-?had no influence on the expression of AdipoR mRNA.

Expression of adiponectin mRNA and related factors in greater omentum fat tissue in obese subjects / 中华内分泌代谢杂志

The expression of adiponectin mRNA in greater omentum fat tissue was measured in 43 subjects using RT-PCR.The results showed that the expression of adiponectin mRNA in greater omentum fat tissue was significantly lower in obese subjects than that in normal persons,and abdominal obesity,HOMA-IR and tumor necrosis factor-?were the main independent factors influencing the expression of adiponectin mRNA in greater omentum.

Effects of TNF-?on PPAR-?2 mRNA expression and adiponectin secretion in 3T3-L1 adipocytes / 中华内分泌代谢杂志

Undifferentiated and differentiated 3T3-L1 adipocytes were treated with 100 ng/ml tumor necrosis factor-?(TNF-?),and peroxisome proliferator-activated receptor-?2 (PPAR-?2) mRNA expression and adiponectin secretion in cultured cells were measured.The results showed that TNF-?suppressed PPAR-?2 mRNA expression and adiponeetin secretion in 3T3-L1 adipocytes (P