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Purpose@#Triple-negative breast cancer (TNBC) is highly malignant and has poor prognosis and a high mortality rate. The lack of effective therapy has spurred our investigation of new targets for treating this malignant cancer. Here, we identified RON (macrophage-stimulating 1 receptor) and MET (MET proto-oncogene, receptor tyrosine kinase) as a prognostic biomarker and therapeutic targets for potential TNBC treatment. @*Materials and Methods@#We analyzed RON and MET expression in 187 primary TNBC clinical samples with immunohistochemistry. We validated the targeted therapeutic effects of RON and MET in TNBC using three tyrosine kinase inhibitors (TKIs): BMS-777607, INCB28060, and tivantinib. The preclinical therapeutic efficacy of the TKIs was mainly estimated using a TNBC xenograft model. @*Results@#Patients with TNBC had widespread, abnormal expression of RON and MET. There was RON overexpression, MET overexpression, and RON and MET co-overexpression in 63 (33.7%), 63 (33.7%), and 43 cases (23.0%), respectively, which had poor prognosis and short survival. In vivo, the TKI targeting RON ant MET inhibited the activation of the downstream signaling molecules, inhibited TNBC cell migration and proliferation, and increased TNBC cell apoptosis; in the xenograft model, they significantly inhibited tumor growth and shrank tumor volumes. The TKI targeting RON and Met, such as BMS-777607 and tivantinib, yielded stronger anti-tumor effects than INCB28060. @*Conclusion@#RON and MET co-overexpression can be significant pathological characteristics in TNBC for poor prognosis. TKIs targeting RON and MET have stronger drug development potential for treating TNBC.
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Objective To evaluate the noninferiority of domestic electomyographic (EMG) evoked potential instrument utility on avoiding iatrogenic injury of nerve roots compare to the imported ones,and understand the methods,accuracy and practicability of domestic machine on monitoring nerve root at the fracture fixation process.Methods Domestic of Neurocare-C machine and Nicolet Viking select8 concurrent monitoring 30 cases of bone fracture fixation,including 20 cases of spinal fixation and 10 cases of fractures fixation of limbs and pelvic.The trend of positive change,the rate and amplitude variation of electromyographic response between two instruments were compared.Results The nerve root function could be accurately judged by EMG monitoring during operation.Intraoperative EMG could be detected by both domestic and imported instruments occurred in 5 patients,3 cases were internal fixation of vertebral fracture,1 case was humeral fractures fixation,1 case was pelvic fracture.The rate of electromyographic response of both domestic and imported instruments were 16.67％.There was no significant difference in amplitude variation of electromyographic response of quadriceps femoris,biceps femoris,rectus femoris and tibialis anterior between two instruments (P ＞ 0.05).Conclusion Both domestic and imported EMG evoked potentials can accurately and timely respond to nerve root function,avoid nerve root injury,and ensure the safety of fracture fixation surgery.
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Objective To observe the clinical efficacy of Shugan Hewei Formula for cancer-related depression. Methods Totally 92 cancer-related depression patients were randomly divided into two groups, with 46 patients in each group. All patients already finished the treatment such as radiotherapy and chemotherapy, and were in stable stage. The Fluoxetine Hydrochloride Dispersible Tablets was given to the patients in the control group, 20 mg each time,once a day,orally;Shugan Hewei Formula was given to those in the treatment group,a dose a day,twice a day, orally. The treatment for both groups lasted for 6 weeks. Before and after treatment, HAMD scores and KPS scores were observed; the change of cerebral neurotransmitters levels in the brain of patients were detected; IL-6 and TNF-α in serum were tested by ELISA. Results HAMD scores in the two groups decreased markedly after treatment (P<0.05). HAMD scores in treatment group was less than that in control group after treatment (P<0.05). KPS scores increased in treatment group after treatment, and were better than the control group (P<0.05). The contents of NE, 5-HT increased, IL-6 and TNF-αdecreased in both groups after treatment (P<0.05). The contents of NE and 5-HT in the treatment group were higher than the control group after treatment, while the contents of IL-6 and TNF-α were lower than the control group (P<0.05, P<0.01). No adverse reactions and side effects were observed in the treatment group. 5 cases of mild nausea and 6 cases of abnormal liver function were found in the control group. Conclusion Shugan Hewei Formula can increase the contents of NE and 5-HT in the brain of cancer-related depression patients, decreased cytokines levels, and improve patients' depressive state.
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Objective To observe the clinical efficacy of Shugan Hewei Formula for cancer-related depression. Methods Totally 92 cancer-related depression patients were randomly divided into two groups, with 46 patients in each group. All patients already finished the treatment such as radiotherapy and chemotherapy, and were in stable stage. The Fluoxetine Hydrochloride Dispersible Tablets was given to the patients in the control group, 20 mg each time,once a day,orally;Shugan Hewei Formula was given to those in the treatment group,a dose a day,twice a day, orally. The treatment for both groups lasted for 6 weeks. Before and after treatment, HAMD scores and KPS scores were observed; the change of cerebral neurotransmitters levels in the brain of patients were detected; IL-6 and TNF-α in serum were tested by ELISA. Results HAMD scores in the two groups decreased markedly after treatment (P<0.05). HAMD scores in treatment group was less than that in control group after treatment (P<0.05). KPS scores increased in treatment group after treatment, and were better than the control group (P<0.05). The contents of NE, 5-HT increased, IL-6 and TNF-αdecreased in both groups after treatment (P<0.05). The contents of NE and 5-HT in the treatment group were higher than the control group after treatment, while the contents of IL-6 and TNF-α were lower than the control group (P<0.05, P<0.01). No adverse reactions and side effects were observed in the treatment group. 5 cases of mild nausea and 6 cases of abnormal liver function were found in the control group. Conclusion Shugan Hewei Formula can increase the contents of NE and 5-HT in the brain of cancer-related depression patients, decreased cytokines levels, and improve patients' depressive state.