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1.
Chinese Journal of Tissue Engineering Research ; (53): 2039-2044, 2018.
Artigo em Chinês | WPRIM | ID: wpr-698655

RESUMO

BACKGROUND: Studies have confirmed that monosialotetrahexosyl ganglioside can induce human umbilical cord mesenchymal stem cells to differentiate into neuron-like cells, but little is reported on its optimal concentration. OBJECTIVE: To explore the optimal concentration of monosialotetrahexosyl ganglioside that induces human umbilical cord mesenchymal stem cells to differentiate into neuron-like cells in vitro. METHODS: Human umbilical cord mesenchymal stem cells were isolated by using collagenase digestion method, and after expansion, passage 3 cells were randomly allocated into five groups. When 70%-80% of cells were confluent, 50, 100, 150 and 200 mg/L monosialotetrahexosyl ganglioside induction solutions were added in corresponding experimental groups, while cells in the blank control group were cultured in the same volume of L-DMEM medium. Cell morphology was observed under inverted phase contrast microscope. Expression levels of microtubule-associated protein 2, neurofilament protein and glial fibrillary acidic protein were measured by using immunohistochemistry at 6 hours after induction. RESULTS AND CONCLUSION: Human umbilical cord mesenchymal stem cells were isolated successfully and sub-cultured stably. These cells could express surface markers of mesenchymal stem cells. Monosialotetrahexosyl ganglioside at the optimal concentration of 150 mg/L was confirmed to induce the neuron-like differentiation of human umbilical cord mesenchymal stem cells, and differentiated cells could express microtubule-associated protein 2 and neurofilament protein as neuron-specific markers.

2.
Chinese Medical Journal ; (24): 1616-1622, 2011.
Artigo em Inglês | WPRIM | ID: wpr-353996

RESUMO

<p><b>BACKGROUND</b>Intensive blood glucose control is proven to be associated with the diabetic microvascular and macrovascular complications, which could affect quality of life (QOL). This study was performed to determine the effects of intensive glucose control therapy on QOL of elderly patients with type 2 diabetes in Anhui Province.</p><p><b>METHODS</b>Ninety-seven elderly patients with type 2 diabetes in Anhui were randomly assigned to standard treatment group and intensive therapy group. All patients were followed up for five years on average. Correlated information has been collected during the regular follow-up.</p><p><b>RESULTS</b>Patients with microvascular complications reported significantly lower European Quality of Life-5 Dimensions (EQ-5D) scores and had more problems with usual activities, pain and anxiety than those without complications (P < 0.05). Patients having experienced hypoglycemic episodes had significantly more problems with anxiety than those without hypoglycemic episodes (P < 0.05). No significant difference was detected in all dimensions in quality of life, as well as in Visual Analog Scale score between two groups (P > 0.05). There was no significant difference in quality of life at the fifth year compared with that of the first year in both groups. Women had more feelings of pain and anxiety than men (P < 0.05) and longer disease course was associated with increased levels of pain and anxiety (P < 0.05), as well as with lower QOL. In addition, patients with higher body mass index (BMI) had more problems with daily activities than patients with lower BMI (P < 0.05).</p><p><b>CONCLUSIONS</b>Anxiety is common in elderly diabetic patients and they experienced frequent hypoglycemic episodes. Diabetic vascular complications significantly affect QOL of the patients. Intensive glucose control has no significant effect on QOL of the diabetic patients. Female, older age, long disease course, less education and high BMI are all factors caused reduced QOL and patients with these factors should be given more psychological support. Frequent mild hypoglycemic episodes do not cause impaired function of the central nervous system.</p>


Assuntos
Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Glicemia , Metabolismo , Diabetes Mellitus Tipo 2 , Sangue , Jejum , Sangue , Hemoglobinas Glicadas , Metabolismo , Qualidade de Vida
3.
Journal of Central South University(Medical Sciences) ; (12): 149-152, 2005.
Artigo em Chinês | WPRIM | ID: wpr-813416

RESUMO

OBJECTIVE@#To determine the effect of ginsenoside on the cellular proliferation, apoptosis and cell cycles in LC A549 and HUVEC 304 cell lines.@*METHODS@#A549 and HUVEC 304 cell lines were cultured with different concentrations of ginsenoside. Cellular proliferation was detected with MTT, apoptosis and cell cycles were checked with Flow Cytometer, and change of microstructure was observed by transmission electron microscope.@*RESULTS@#The apoptosis rate was 29.8% in A549 cell lines after being interfered with ginsenoside at 3 x 10(-6) mol/L, significantly higher than that in the control group ( P < 0.05). No change was observed in the cell cycles after being interfered with ginsenoside. The inhibitive rate of ginsenoside was 12.53% for HUVEC 304 cell line at 1 x 10(-4) mol/L (P < 0.05 ). The cells induced by conditioned medium could be inhibited by ginsenoside, and apoptotic body could be found in cells induced by conditioned medium at 10(-6) mol/L.@*CONCLUSION@#The proliferation of vascular endothelial cell could be inhibited by ginsenoside, and apoptosis could also be found in both tumor cells and cells induced by conditioned medium after being interfered with ginsenoside.


Assuntos
Humanos , Adenocarcinoma , Patologia , Antineoplásicos Fitogênicos , Farmacologia , Apoptose , Ciclo Celular , Linhagem Celular , Linhagem Celular Tumoral , Proliferação de Células , Células Endoteliais , Biologia Celular , Ginsenosídeos , Farmacologia , Neoplasias Pulmonares , Patologia , Veias Umbilicais , Biologia Celular
4.
Chinese Journal of Medical Genetics ; (6): 698-701, 2005.
Artigo em Chinês | WPRIM | ID: wpr-279964

RESUMO

<p><b>OBJECTIVE</b>To investigate the distribution of SNP276 in adiponectin gene in Chinese Hans and its impact on type 2 diabetes mellitus and insulin sensitivity.</p><p><b>METHODS</b>The study population consisted of 417 Chinese Hans residents in Anhui province, including 141 subjects with normal glucose tolerance (NGT) and 276 with type 2 diabetes (T2DM). The islet beta-cell insulin secretion and tissue insulin sensitivity were assessed by formulae of homeostasis model assessment (HOMA-IR & HOMA beta). Firstly, polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was used to determine whether variation exists in APM1. Then, exact variation was detected by automated DNA direct sequencing.</p><p><b>RESULTS</b>The genotypes of APM1 SNP276 were 0.489 GG, 0.418 GT and 0.092 TT and the major allele was G (frequency=0.699) in subjects with NGT. The distributions of genotypes and alleles of SNP276 both displayed significant difference between NGT and T2DM groups (P=0.031 and 0.013). The SNP276 non-TT (TG+GG) genotype was associated with increased risk of T2DM (OR=2.447, 95%CI: 1.067-5.612, P=0.035). In T2DM group, the subjects with SNP276 GG or GT genotype had higher body mass index, body fat content, fasting plasma glucose and HOMA-IR than did those with TT genotype (P < 0.05 or P < 0.01). Besides, GG genotype had higher systolic blood pressure (P=0.021). In NGT group, SNP276 non-TT carrier had increased body mass index, body fat content, waist hip ratio, fasting plasma insulin, oral glucose tolerance test 2 h plasma insulin and HOMA-IR when compared with TT genotype (P < 0.05 or 0.01).</p><p><b>CONCLUSION</b>SNP276 in APM1 was associated with T2DM and insulin sensitivity.</p>


Assuntos
Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adiponectina , Genética , Sequência de Bases , Glicemia , Metabolismo , Diabetes Mellitus Tipo 2 , Sangue , Genética , Frequência do Gene , Predisposição Genética para Doença , Genética , Genótipo , Insulina , Sangue , Resistência à Insulina , Reação em Cadeia da Polimerase , Polimorfismo de Nucleotídeo Único , Polimorfismo Conformacional de Fita Simples , Análise de Sequência de DNA
5.
China Journal of Chinese Materia Medica ; (24): 357-360, 2005.
Artigo em Chinês | WPRIM | ID: wpr-279163

RESUMO

<p><b>OBJECTIVE</b>To study the effect of 20( R)-ginsenoside Rg3 on the expressions of angiogenesis factors proteins (VEGF,bFGF, MMP-2) in human lung adenocarcinoma cell line A549 and HUVEC304 cell.</p><p><b>METHOD</b>The cell lines of A549 and HUVEC304 were cultured with 20(R)- Rg3. The gray scale and positive rate of VEGF, bFGF, MMP-2 were detected by immunohistochemistry. The differential expressions of genes were studied by DNA microarray.</p><p><b>RESULT</b>The positive rate of VEGF protein in A549 cell decreased significantly as compared with the control group ( P = 0.03). The gray scales of VEGF, Flt, KDT proteins in both A549 cell lines and HUVEC 304 cell lines decreased ( P = 0.05). Gray scale of MMP-2 also decreased in A549 cell lines. The result of differential expressions of genes of A549 cell lines showed that 14 genes were down-regulated and 10 genes were up-regulated.</p><p><b>CONCLUSION</b>The Chinese materia medica of 20( R)-Rg3 can inhibit the expression of angiogenesis factors proteins via several target genes in both tumour cell and vascular endothelial cell.</p>


Assuntos
Humanos , Adenocarcinoma , Genética , Metabolismo , Patologia , Linhagem Celular Tumoral , Células Endoteliais , Metabolismo , Perfilação da Expressão Gênica , Ginsenosídeos , Farmacologia , Neoplasias Pulmonares , Genética , Metabolismo , Patologia , Metaloproteinase 2 da Matriz , Metabolismo , Neovascularização Patológica , Análise de Sequência com Séries de Oligonucleotídeos , Panax , Química , Veias Umbilicais , Biologia Celular , Fator A de Crescimento do Endotélio Vascular , Metabolismo
6.
Chinese Journal of Epidemiology ; (12): 534-536, 2004.
Artigo em Chinês | WPRIM | ID: wpr-342317

RESUMO

<p><b>OBJECTIVE</b>To study the association of muscle-specific glycogen-targeting regulatory subunit of the glucogen-bound protein phosphatase 1 (PPP1R3) gene codon 905 Asp/Tyr polymorphism with type 2 diabetes in Chinese Han population in Hefei region of Anhui province.</p><p><b>METHODS</b>PPP1R3 gene Asp905Tyr polymorphism was detected by polymerase chain reaction and appropriate restriction enzyme (PCR-RFLP) in 262 type 2 diabetic cases and 104 normal controls. Case and control groups were divided into subgroups by body mass index (BMI) 25 kg/m2.</p><p><b>RESULTS</b>When PPP1R3 gene Asp905Tyr polymorphism was not associated with type 2 diabetes mellitus. When subjects with BMI < 25 kg/m2 and Tyr/Tyr genotypes were used as reference. Subjects with Asp905 and BMI > or = 25 kg/m2 had a 3.69-fold increase of risk suffering from type 2 diabetes (OR = 3.69, 95% CI: 1.38-8.89, P=0.006).</p><p><b>CONCLUSIONS</b>PPP1R3 gene Asp905Tyr polymorphism did not seem to play a critical role in the development of type 2 diabetes mellitus in Han population of Chinese in Anhui province but interaction between the Asp905 and BMI cause the increase of risk of type 2 diabetes.</p>


Assuntos
Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Alelos , Ácido Aspártico , Genética , China , Epidemiologia , Etnologia , Diabetes Mellitus Tipo 2 , Epidemiologia , Genética , Frequência do Gene , Genótipo , Obesidade , Fosfoproteínas Fosfatases , Genética , Reação em Cadeia da Polimerase , Polimorfismo Genético , Polimorfismo de Fragmento de Restrição , Proteína Fosfatase 1 , Fatores de Risco , Tirosina , Genética
7.
Chinese Journal of Medical Genetics ; (6): 29-31, 2004.
Artigo em Chinês | WPRIM | ID: wpr-329406

RESUMO

<p><b>OBJECTIVE</b>To determine whether the muscle-specific glycogen-targeting regulatory subunit of the glucogen bound protein phosphatase 1 (PPP1R3) gene 5 bp deletion/insertion(D/I) within 3'-untranslated region ( 3'-UTR) polymorphism is associated with type 2 diabetes in Chinese Han population in Hefei region of Anhui province.</p><p><b>METHODS</b>The PPP1R3 gene 3'-UTR 5 bp D/I polymorphism was detected by polymerase chain reaction in 268 patients with type 2 diabetes and 106 normal controls.</p><p><b>RESULTS</b>(1) The distributions of the frequency of three genotypes and two alleles of the PPP1R3 gene 5 bp D/I polymorphism showed no significant difference between the type 2 diabetic cases and the normal controls. (2) In both the cases and controls, there was no significant difference in age at onset, duration of disease, blood glucose, blood lipid profile, blood pressure, insulin sensitive index, body mass index, and waist hip ratio between the three genotypic groups(P 0.05). (3) The PPP1R3 gene 3'-UTR polymorphism in Chinese Han population in Hefei region of Anhui province was found to be similar to that in both Japanese population and Canadian population, and to be different from that in Piman Indians and the Caucasians in Sweden.</p><p><b>CONCLUSION</b>The PPP1R3 gene 5 bp D/I within 3'-UTR polymorphism taking on genetic variation among the different races of mankind may not play a critical role in the development of type 2 diabetes mellitus in Chinese Hans of Hefei region in Anhui province.</p>


Assuntos
Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Região 3'-Flanqueadora , Genética , Alelos , Diabetes Mellitus Tipo 2 , Genética , Patologia , Frequência do Gene , Genótipo , Mutagênese Insercional , Fosfoproteínas Fosfatases , Genética , Polimorfismo Genético , Proteína Fosfatase 1 , Deleção de Sequência
8.
Chinese Journal of Endocrinology and Metabolism ; (12)2000.
Artigo em Chinês | WPRIM | ID: wpr-676241

RESUMO

The expression of adiponectin mRNA in greater omentum fat tissue was measured in 43 subjects using RT-PCR.The results showed that the expression of adiponectin mRNA in greater omentum fat tissue was significantly lower in obese subjects than that in normal persons,and abdominal obesity,HOMA-IR and tumor necrosis factor-?were the main independent factors influencing the expression of adiponectin mRNA in greater omentum.

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