Simultaneous determination of four glycosylflavones from Lophatherum gracile by RP-HPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To develop a RP-HPLC method for simultaneous determination of orientin, isorientin, vitexin and isovitexin in Lophatherum gracile from different habitat and harvesting time.</p><p><b>METHOD</b>The HPLC method was applied and the chromatographic column was a Waters XBridge C18 column (4.6 mm x 250 mm, 5 microm). The mobile phase consisted of methanol-0.05% acetic acid (35:65). The flow rate was 1.0 mL min(-1) and the detection wavelength was set at 340 nm. The column temperature was set at 25 degrees C.</p><p><b>RESULT</b>Four components were isolated well, the linear relationships were excellent. The mean recoveries and RSD values of orientin, isorientin, vitexin and isovitexin were 103.2%, 2.1%; 101.6%, 2.7%; 98.4%, 2.3%; 99.2%, 1.8%, respectively.</p><p><b>CONCLUSION</b>The HPLC method is simple, sensitive and reliable, and can be used for the quality control of the medicinal material.</p>

Chemical constituents of C-glycosylflavones from Mimosa pudica / 药学学报

<p><b>AIM</b>To study chemical constituents of Mimosa pudica of Hainan province.</p><p><b>METHODS</b>The constituents were separated and purified by column chromatography with macroporous adsorption resin Diaion HP-20, Sephadex LH-20, Toyopearl HW-40, MCI Gel CHP-20, RP-18 and normal phase silica gel. Their structures were identified on the basis of physical and spectral data.</p><p><b>RESULTS</b>Four compounds were isolated and identified as: 7, 8, 3', 4'-tetrahydroxyl-6-C-[alpha-L-rhamnopyranosyl-(1 --> 2)]-beta-D-glucopyranosyl flavone (I); 5, 7, 4'-trihydroxyl-8-C-[alpha-L-rhamnopyranosyl-( --> 2)]-beta-D-glucopyranosyl flavone (II); 5, 7, 3', 4'-tetrahydroxyl-6-C-[alpha-L-rhamnopyranosyl-(1 --> 2)]-beta-D-glucopyranosyl flavone (III); catcher (IV).</p><p><b>CONCLUSION</b>Compound I is a new compound and componuds II - IV were isolated from this plant for the first time.</p>