RESUMO
Secondary lymphoid-tissue chemokine (SLC) is a type of CC chemokine identified by searching the Expressed Sequence Tag (EST) database. The full-length SLC gene was synthesized based on human SLC sequence using SOE-PCR. The sequenced SLC gene was cloned into expression vector pTMF and pALM, which used to transform Escherichia coli. Then the E. coli was cultured and induced according to protocol. The expressed target protein was identified by Western blotting. The target protein was expressed as soluble protein as well as inclusion bodies, the ratio of these two forms target protein varied with the difference conditions of culture and induction. The target protein was purified with the methods of nickel-nitrilotriacetic acid (Ni-NTA) metal-affinity chromatography. The results of electrophoresis of the purified target protein showed that the molecular weight was larger than the predicted molecular weight.
Assuntos
Humanos , Sequência de Bases , Western Blotting , Quimiocina CCL21 , Química , Genética , Metabolismo , Cromatografia de Afinidade , Clonagem Molecular , Eletroforese em Gel de Poliacrilamida , Escherichia coli , Genética , Expressão Gênica , Vetores Genéticos , Genética , Dados de Sequência Molecular , Peso Molecular , Proteínas Recombinantes , Química , Metabolismo , Transformação GenéticaRESUMO
<p><b>OBJECTIVE</b>To compare the clinical therapeutic effects of acupuncture at Jiaji (EX-B 2), Chinese herbs and western medicine on nervous tinnitus.</p><p><b>METHODS</b>Ninety cases were randomly divided into 3 groups, 30 cases in each group. The acupuncture group were treated with acupuncture at cervical Jiaji (EX-B 2), 20 min each session, once a day, 10 sessions constituting one course; the Chinese herbs group with modified Buzhong Yiqi Decoction (decocted in water), one dose each day, 10 doses constituting one course; the western medicine group with bandazol, Dextran 40, Danshen tablet, and vitamin B12, 10 days constituting one course. After 3 courses, the therapeutic effects were evaluated with criteria of assessment for therapeutic effects.</p><p><b>RESULTS</b>The effective rates in the 3 groups were 73.3%, 40.0% and 33.3%, respectively, with significant differences among the 3 groups (P < 0.05).</p><p><b>CONCLUSION</b>Acupuncture has obvious therapeutic effect on nervous tinnitus, and acupuncture at cervical Jiaji (EX-B 2) is an effective therapy for nervous tinnitus, and its therapeutic effect is better than those of Chinese herbs and western medicine.</p>