Generation and preliminary characterization of chimeric antibodies against neuraminidase of H7N9 subtype influenza A virus / 中华实验和临床病毒学杂志

Objective@#To develop the chimeric antibodies against neuraminidase (NA) of H7N9 and to identify their biological activity and function.@*Methods@#The genes of variable regions of the light chain (VL) and heavy chain (VH) obtained by mouse hybridoma technology were cloned respectively into the expression VH and VL vectors bearing human-derived Cγ1, and Cκ1 and co-transfected into 293T cells. The chimeric antibodies were purified and their functions were investigated.@*Results@#Two chimeric antibodies, 1E2 and 3E3 against neuraminidase (NA) of H7N9 were obtained. Both antibodies recognized similar antigenic epitopes. MAb 1E2 and 3E3 could prevent the infectivity with H7N9 and H11N9 virus and reduce their size of viral plaque.@*Conclusions@#The chimeric antibodies specific for N9 could prevent the infection of N9 subtype influenza virus as well as the NAI-resistant mutants and could be a potential immunotherapy approach for H7N9 treatment.

Progress in the mechanism of glycometabolism in memory CD8+ T cell formation / 中华微生物学和免疫学杂志

CD8+T cells are critical immune cells protecting the body against infection and cancer. Long-lived memory CD8+T cells formed in a prior infection can reproduce to mount a faster and stronger im-mune response at a second encounter with the cognate antigen. The activation, clonal expansion and re-sponse of T cells are energetically demanding processes tightly coupled in cellular metabolism. Meanwhile, changes in cellular metabolism could also affect the development of memory T cells following acute infection. In this review, we discussed the current understanding of the mechanism by which glycometabolic pathways manipulate the differentiation of memory CD8+T cells in order to provide reference for improving vaccine de-velopment and cancer treatment.

Research progress in broad neutralizing antibodies and universal vaccines against influenza A virus / 中华微生物学和免疫学杂志

Influenza vaccination is the most effective means to prevent and control influenza epi-demics, and the universal influenza vaccine which can induce broad and long-term protective effect is still in its infancy. Up to date, several broad neutralizing antibodies that can antagonize a variety of influenza A vi-ruses have been identified, and the crystal structure of the antibody and HA complex reveals at least three highly conserved epitopes. Deep insights into the molecular mechanism of the interactions between neutrali-zing antibodies and virus antigen can elicit a new strategy not only for rational design of universal influenza vaccines, but also for the development of antibody-based therapeutics against influenza virus. In this paper, advances in research of universal neutralizing antibodies and vaccines for Influenza A virus are reviewed.

Effect of high fat diet on UCP2 mRNA expression in white adipose tissue of SR-A Ⅰ/Ⅱ gene knock-out mice / 中国病理生理杂志

AIM: To observe the relationship between UCP2 mRNA expression in white adipose tissue and diet-induced obesity in SR-A I/II gene knock-out(SR-AⅠ/Ⅱ-/-) mice.METHODS: Fluorescent quantitative RT-PCR was used to detect UCP2 mRNA expression in mice epididymal white adipose tissue.The cellular morphological changes were analyzed by using image analysis.Serum TG,TC and LDL-C concentrations were measured by enzymatic determination.RESULTS: After fed with high fat diet for 12 weeks,average body weight of SR-A I/II-/-mice was much higher than that of wild type(SR-A I/II+/+) control mice(P