The function of NLRP1 in noninfectious pulmonary injury following allogeneic hematopoietic stem cell transplantation / 中华血液学杂志

Objective@#To explore the function of NLRP1 in noninfectious pulmonary injury (nonIPI) after allogeneic stem cell transplantation (allo-HSCT) .@*Methods@#In this study, we established the model of allo-HSCT with C57BL/6 and NLRP-/- mouse as recipients. Chimera rate was measured by flow cytometry. The HE staining was used to observe the pathology changes in the lungs. NLRP1 and relevant inflammatory proteins were measured by Western Blot.@*Results@#On the day 14 after allo-HSCT, the chimera rate was more than 96%, HSCs of donors had been successfully transplanted into recipients. HE staining showed that nonIPI occurred after allo-HSCT. The degrees of injuries reached the peak on day 21. In addition, the expressions of MPO, NLRP1, p20, Mature-IL-1β and Mature-IL-18 had same tends with the degrees of nonIPI. When we knocked out NLRP1 gene of recipients, the degrees of nonIPI reduced and the expressions of MPO, p20, Mature-IL-1β and Mature-IL-18 were less than in non-knockout group.@*Conclusion@#allo-HSCT could cause nonIPI and high expressions of MPO, p20, IL-1β, IL-18, NLRP1. Knocking out NLRP1 gene could alleviate the degrees of nonIPI and reduce the expressions of relevant inflammatory proteins, indicating that NLRP1 might be one of factors contributed to nonIPI after allo-HSCT.

Effects of allogeneic hematopoietic stem cell transplantation in combination with infusion of endothelial progenitor cells on bone marrow inflammatory injury / 中华血液学杂志

Objective@#To explore effects of allogeneic hematopoietic stem cell transplantation (HSCT) in combination with infusion of endothelial progenitor cells (EPC) on bone marrow inflammatory injury.@*Methods@#6-8 weeks BALB/c (H-2Kd) mice after lethal dose of irradiation (TBI) were subjected to allogeneic bone marrow transplantation (BMT group) or co-transplantation of EPC (EPC group) . Samples of bone marrow cells of mice in each group on days 7,14,21,28 after transplantation were obtained to detect EPC cultural and cell chimeric rates by flow cytometer. Mice were sacrificed on days 7, 14, 21 and 28 post HSCT to analyze bone marrow pathology by H&E staining, the infiltration of macrophages and neutrophils by Western blot, validation expression levels of inflammatory complexes nlrp1、nlrp6 and its downstream molecules casepase-1 by Q-PCR and Western blot.@*Results@#Cell chimeric rate on day 7 after transplantation in EPC group[ (91.65±2.77) %] was significantly higher than in BMT group[ (83.69±1.26) %]. Alleviated osteomyelitis injury and inflammatory cell infiltration in EPC group were observed when compared with BMT mice. Also significant reductions of the levels of nlrp1、nlrp6、casepase-1 transcription complexes in EPC mice were noted when compared with BMT ones.@*Conclusion@#Co-transplantation of HSC and EPC could alleviate inflammatory cell infiltration and activation of the complex to promote the repair of bone marrow.

Evaluation of the perioperative period safety of improved transurethral plasma kinetic enucleation of prostate in high-risk benign prostatic hyperplasia patients with coronary heart disease / 中国医师进修杂志

Objective To evaluate the perioperative period safety of improved transurethral plasma kinetic enucleation of prostate (TUPKEP) in high-risk benign prostatic hyperplasia (BPH) patients with coronary heart disease (CHD).Methods One hundred and twenty-eight BPH patients were selected,24 patients had CHD (with CHD group),among whom 10 patients were given transurethral vapor-resection of prostate (TUVP),and 14 patients were given improved TUPKEP; 104 patients didn't have CHD,among whom 22 patients were given TUVP,and 82 patients were given improved TUPKEP.The serum endothelin (ET)-1 was measured by specific radioimmunoassay at preoperative 2 h and postoperative 1,2,6 d,and complication was observed.Results All the patients were cured by operation,and left hospital smoothly.There were no statistical differences in the preoperative 2 h serum ET-1 in with CHD group and without CHD group (including all TUVP patients and improved TUPKEP patients) (P ＞ 0.05).The postoperative 1 and 2 d serum ET-1 levels of TUVP patients were significantly higher than those of improved TUPKEP patients,in with CHD group:(114.09 ± 15.33) ng/L vs.(94.77 ± 12.14) ng/L and (99.67 ± 9.87) ng/L vs.(88.21 ± 9.55) ng/L; in without CHD group:(70.21 ± 12.44) ng/L vs.(53.67 ± 9.02) ng/L and (61.18 ± 9.52) ng/L vs.(48.54 ± 9.15) ng/L,and there were statistical differences (P ＜ 0.05).There were no statistical differences in postoperative 6 d serum ET-1 in TUVP patients and improved TURKEP patients (P ＞ 0.05).In with CHD group,5 patients had ischemic ST-T change in the early postoperative period,and 3 patients had angina pectoris.They all were promptly treated,and the events were controlled.Serious complications did not present such as acute myocardial infarction (AMI),acute heart failure and sudden cardiac death,etc.Conclusions The postoperative BPH patients have vascular endothelial injury catholically,especially the high-risk patients with CHD.Furthermore,it might be one of the causes of the postoperative adverse cardiovascular events.Compared with TUVP,improved TUPKEP has a minor impact on vascular endothelial function,and it can reduce the postoperative adverse cardiovascular events in the BPH patients with CHD.Improved TUPKEP is a relatively safer surgical method for high-risk BPH with CHD.

Resources collocation and management for medical relief in quake-stricken disaster region / 中华急诊医学杂志

This in a survey of collocation and administration of medical supplies during Wenchuan earthquake. (1) Material utilized for trauma wound: in the early phase after Wenchuan earthquake, lots of trauma patients needed materials for hemostasis and debridement such as gauze,bandage and wooden plate. (2) Material for isolation and protective care: the wound began to be infected with the prolonged heeling, so isolation was needed and plenty of material was reqired for prevention of cross infection at this time, such as disposable isolation gowns. (3)First-aid boxes for transfer; material used for receiving patients from disaster areas at airport; material used for transfering patients from medical care units of disaster areas, and material needed for transfering patients to other provinces by train or by air. Three was a set of 3 first-aid boxes, one for medicine such as adrenalin, atropine, doamine or aramine,other two boxes for suction machine for removal of secretion, ECG monitor and intubation apparatus. According to the experience from the Wenchuan earthquake,we put forth a suggestion that emergency equipments should be divided into A,B,and C categories for reserve in peacetime. Type A is essential equipment including a variety of small appliances and apparatus. Type B is the medicinal substances including first-aid medicine,disinfection agents and so on, which should be replaced regularly in terms expiration date. Type C is consumable stuff. The different combinations between type A and type B or type C can be used in different circumstance for different purpose.

RANDOMIZED DOUBLE-BLIND CLINICAL TRIAL OF THE ANTIHYPERTENSIVE EFFICACY OF BISOPROLOL,ENALAPRIL AND NIFEDIPINE RETARD ON PATIENTS IN THE TROOPS IN DIFFERENT GEOGRAPHICAL REGIONS / 解放军医学杂志

0 05). For enalapril treatment, the total efficacy rate in patients in Tibet and coastal areas was 96 0% and 77 6%( P 0 05).