Expression of Th17 and CD4+ CD25+ T regulatory cells in peripheral blood of acute leukemia patients and their prognostic significance

To discuss the expression of T helper cell 17 [Th17] cells and CD4[+] CD25[+] Foxp3[+] regulatory T cells [Treg] in peripheral blood [PB] of patients with acute leukemia [AL], and to explore the relationship between them and disease prognosis. 40 patients diagnosed with acute leukemia in The First Affiliated Hospital of Zhengzhou University from July 2012 to August 2014 were selected as the observation group. Meanwhile, 40 healthy people were taken as the control group. Flow Cytometry Method [FCM] was used to detect the level of Th17 cells and CD4[+] CD25[+] Foxp3[+] cells in peripheral blood of the two groups, and enzyme-linked immuno sorbent assay [ELISA] method was used to test the level of IL17 and TGF-beta in peripheral blood of two groups; reverse transcription-polymerase chain reaction [RT-PCR] was adopted to analyze the mRNA levels of ROR gamma T and Foxp3 in peripheral blood. In addition, we examined the levels of Th17 and CD4[+] CD25[+] Foxp3[+] cells and associated factor levels in patients with remission after AL chemotherapy. the Th17 cells [CD3+ CD4+ IL-17+] in acute leukemia patients accounted for [1.51+/-0.27]%, which was significantly higher than that of control group [0.36+/-0.23]%, with statistical significance [t=20.51, P<0.001]; the percentage of CD4[+] CD25[+] Foxp3[+] cells in AL patients was [3.37+/-0.48]%, which was significantly higher than that of control group of [1.26+/-0.27]%, with statistical significance [t=24.23, P<0.001]; the serum levels of IL-17 and TGF-beta in AL patients were [28.12+/-6.33] pg/ml and [38.41+/-8.44] pg/ml respectively, which were all significantly higher than that of control group of [14.41+/-6.21] pg/ml and [24.49+/-7.42] pg/ml, with statistical significance [t=7.83, P<0.001; t=7.83, P<0.001]; the ROR gamma T mRNA and Foxp3 mRNA levels in AL patients were all significantly higher than that of control group, with statistical significance [t=12.27, P<0.001; t=7.89, P<0.001]. In addition, compared with before chemotherapy, the levels of Th17 cells and CD4[+] CD25[+] Foxp3[+] cells, and the serum levels of IL-17 and TGF-beta in acute leukemia patients all decreased significantly after 6 months of chemotherapy, and the difference was statistically significant [P<0.001]. Th17 cells, CD4[+] CD25[+] Foxp3[+] cells and their secretory proteins IL-17, TGF-beta and transcription factors were significantly increased in AL patients. Therefore, regular detection of peripheral blood Th17 and Treg cells, as well as their secretory proteins are useful for monitoring the immune status and prognosis of patients

Causes and nursing countermeasures in pediatric PICC catheter complications

To analyze the complications and nursing countermeasures of PICC [Peripherally Inserted Central Catheter] catheters. using children PICC catheter technique 40 cases, complications were observed, and analyze the original causes, in order to propose a solution. There were 10 cases of catheter blockage, 5 cases of catheter infection, 6 cases of phlebitis, 5 cases of puncture difficulties, 2 cases of poor feeding tube, 2 cases of bleeding puncture site. with the continuous exploration and research of nursing intervention, the production of clinical complications from PICC has been used in children were greatly reduced

Expression of Th17 and CD4[+] CD25[+] T regulatory cells in peripheral blood of acute leukemia patients and their prognostic significance

To discuss the expression of T helper cell 17 [Th17] cells and CD4[+] CD25[+] Foxp3[+] regulatory T cells [Treg] in peripheral blood [PB] of patients with acute leukemia [AL], and to explore the relationship between them and disease prognosis. 40 patients diagnosed with acute leukemia in The First Affiliated Hospital of Zhengzhou University from July 2012 to August 2014 were selected as the observation group. Meanwhile, 40 healthy people were taken as the control group. Flow Cytometry Method [FCM] was used to detect the level of Th17 cells and CD4[+] CD25[+] Foxp3[+] cells in peripheral blood of the two groups, and enzyme-linked immuno sorbent assay [ELISA] method was used to test the level of IL17 and TGF-beta in peripheral blood of two groups; reverse transcription-polymerase chain reaction [RT-PCR] was adopted to analyze the mRNA levels of ROR gamma T and Foxp3 in peripheral blood. In addition, we examined the levels of Th17 and CD4[+] CD25[+] Foxp3[+] cells and associated factor levels in patients with remission after AL chemotherapy. The Th17 cells [CD3[+] CD4[+] IL-17[+]] in acute leukemia patients accounted for [1.51 +/- 0.27]%, which was significantly higher than that of control group [0.36 +/- 0.23]%, with statistical significance [t=20.51, P<0.001]; the percentage of CD4[+] CD25[+] Foxp3[+] cells in AL patients was [3.37 +/- 0.48]%, which was significantly higher than that of control group of [1.26 +/- 0.27]%, with statistical significance [t=24.23, P<0.001]; the serum levels of IL-17 and TGF-beta in AL patients were [28.12 +/- 6.33] pg/ml and [38.41 +/- 8.44] pg/ml respectively, which were all significantly higher than that of control group of [14.41 +/- 6.21] pg/ml and [24.49 +/- 7.42] pg/ml, with statistical significance [t=7.83, P<0.001; t=7.83, P<0.001]; the ROR gamma T mRNA and Foxp3 mRNA levels in AL patients were all significantly higher than that of control group, with statistical significance [t=12.27, P<0.001; t=7.89, P<0.001]. In addition, compared with before chemotherapy, the levels of Th17 cells and CD4[+] CD25[+] Foxp3[+] cells, and the serum levels of IL-17 and TGF-beta in acute leukemia patients all decreased significantly after 6 months of chemotherapy, and the difference was statistically significant [P<0.001]. Th17 cells, CD4[+] CD25[+] Foxp3[+] cells and their secretory proteins IL-17, TGF-beta and transcription factors were significantly increased in AL patients. Therefore, regular detection of peripheral blood Th17 and Treg cells, as well as their secretory proteins are useful for monitoring the immune status and prognosis of patients

Effect of different types of counterion on the electronic structure of an antitumor polyoxomolybdate Mo7O246- / 生物医学工程学杂志

The electronic structure of a Lindqvist type [Mo7O24]6- anion with 5 different types of counterion (K+, Na+, NH4+, [NH3Pr]+ and [NH3Pri]+) was calculated by using the Discrete Variational Method coupled with Density Functional Theory (DFT-DVM). It could be concluded through variance analysis to the calculated results that the type of counterion does not influence remarkably the electronic structure of [Mo7O24]6- anions. Perhaps it can be used to explain the experiment fact that the polyoxomolybdate structure of the Mo7O24 framework is apparently of critical significance to antitumor action. On these grounds we forecast that two other lindqvist type heptamolybadates(their counterions are Na+ and [NH3Pr]+ respectively) may also exhibit antitumor activities.