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Objective To investigate the application values of 3 kinds of crossmatching method saline medium method,polybrene test and microcolumn gel test in the neonatal blood transfusion security.Methods 174 newborns needing blood transfusion were simultaneously performed the isotype crossmatching by the saline method,polybrene test and microcolumn gel test.The irregular antibody and the direct antiglobulin test were routinely conducted,the samples with positive irregular antibody were further per-formed the antibody specificity identification.Results 14 cases (8.05%)of crossmatching incompatibility were found by the saline method,1 case (0.57%)by the polybrene test and 62 cases (35.63%)by the microcolumn gel test;among which,31 cases were secondary side crossmatching incompatibility caused by positive direct antiglobulin test,1 was the incompatibility of 3 methods caused by anti-D antibodies.The difference between the polybrene test and the microcolumn gel test was of statistical significance (P <0.05).Conclusion The blood crossmatching by the microcolumn gel test in blood transfusion of newborns requires quite few amount of blood sample,is simple to operate and easy to be standardized.With results clearly and easily measured and sensitivity higher than that of polybrene test,its result is clear and easy to be judged,its sensitivity is higher than that of the polybrene test, which has the important significance for ensuring the neonatal blood transfusion safety.
RESUMO
Objective To investigate the protective effects of alpha-lipoie acid (ALA) against beta-cell damage in streptozotocin (STZ)-induced diabetes in rats. Methods Thirty SD rats were randomly divided into three groups: normal control (NC) group, STZ group and ALA + STZ group, with 10 rats in each group. mg/kg, intraperitoneal injection), till the end of the study (4 weeks later). Blood glucose were measured every 3 days after STZ injection. Malondialdehyde (MDA) and reduced glutathione (GSH) levels were measured in pancreatic homogenates. Pancreatic beta-cells were examined by immunohistocbemical methods, Results STZ induced a significant increase of the level of blood glucose. Body weight of rats in ALA + STZ group was (341±26)g, which significantly lower than (368±3)g in NC group, and high than (301±2)g in STZ group with stas(P < 0. 05). Meanwhile the MDA levels in STZ group and NC group were(1.22 ± 0. 14) and(0.57 ± 0.04)nmoL/mg prot, respectively, and there was significant difference between the two groups (P < 0.05) ; the GSH levels in STZ group and NC group were(16.54 ± 1.10) and(25.46 ± 0.62) mg/g prot (P < 0.05), respectively; degeneration of islet cells and decreased blood glucose were observed in STZ + ALA-pretreated rats; MDA level in pancreatic homogenates was(0.72 ± 0. 23)nmoL/mg prot, which was significantly lower than that in STZ group (P < 0.05) ; the GSH level was (35.33 ± 2.66) mg/g prot, which was significantly higher than that in STZ group (P < 0.05) ; increased staining of insulin and preservation of islet ceils functions were more obvious in the STZ + ALA-pretreated rats. Conclusions ALA exerted its protective effect through reducing the oxidative stress and preserving pancreatic beta-cell integrity.