RESUMO
Objective:To investigate the relationship of manganese superoxide dismutase (MnSOD) gene Val16Ala polymorphism with the occurrence and progression of breast cancer in Hainan province.Methods:Two hundred and two breast cancer patients who were admitted to the Affiliated Hospital of Hainan Medical College from January 2014 to December 2015 and 184 healthy female volunteers (control group) who underwent the physical examination during the same period were selected. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the MnSOD gene Val16Ala polymorphism, and the correlations of different alleles and genotypes with occurrence of breast cancer were compared.Results:The frequency of Ala alleles in the experimental group and control group was 15.3% (62/404) and 13.9% (51/368), respectively. There was no statistical difference in the distributions of 2 alleles and 3 genotypes Val/Val, Val/Ala and Ala/Ala between the experimental group and the control group (all P > 0.05). There was no statistical difference in the frequency of 2 alleles and 3 genotypes before and after menopause between the experimental group and the control group (all P > 0.05). Ala alleles were associated with lymph node metastasis (OR = 1.79, 95% CI 1.04-3.08, χ 2 = 4.457, P < 0.05) and TNM stage (OR = 1.95, 95% CI 1.11-3.41, χ 2 = 5.657, P < 0.05). Conclusion:MnSOD gene Val16Ala polymorphism has no correlation with the occurrence of breast cancer in Hainan province, but it may play a role in promoting tumor progression.