Effect of dachaihutang on expression of carnitine patmitoryl transferase-1 in vascular smooth muscle layer of atherosclerotic rabbits / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the therapeutic effect of Dachaihutang on the development of atherosclerosis (AS) in rabbits and its possible mechanism by detecting the expression level of carnitine patmitoyl transferase-1 (CPT-1) in vascular smooth muscle layer of atherosclerotic rabbits, and search the new way and evidence for AS cures.</p><p><b>METHOD</b>Thirty six male New Zealand white rabbits were divided randomly into control group, model control group, simvastatin group and Chinese traditional medicine dachaihutang group. After 9 weeks and 20 weeks of treatment, serum total cholesterol (TC) and triglyceride (TG) and low-density lipoprotein (LDL) levels were examined. At the end of 25 th weeks, histological changes in ascending aorta were studied by HE staining and histomorphometric analysis. The gene expression of CPT-1 in vascular smooth muscle layer of thoracic aorta was detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULT</b>Compared with model control group, in dachaihutang group serum TC and TG and LDL levels attenuated. Pathomorphology indicated that intima and media (I + M) became thinned, and the ratios of the thickness of intima to media(I/M) and the area of intima to media (SI/SM) were decreased (P < 0.05). Aortic intimal proliferation in Dachaihutang group was associated with a marked increase in CPT-1 expression in vascular smooth muscle layer of thoracic aorta. Compared to simvastatin group, except TG value, other values were higher in Dachaihutang group, however, there were no significant differences between the two groups.</p><p><b>CONCLUSION</b>These findings suggest that early treatment with Dachaihutang not only induces a significant regression of arterial lesions of high cholesterol diet rabbits, but also has a crucial inhibited genesis and development of atherosclerosis effect by up-regulating CPT-1 expression in vascular smooth muscle layer.</p>

Determination of Trace Nickel in Human Hair by Kinetic Fluorimetry / 环境与健康杂志

Objective To establish a new kinetic fluorimetry method for the determination of trace nickel in human hair samples. Methods The method was based on the catalysis of nickel on the oxidation of fluorescein(CF) by hydrogen peroxide in an almost neutral medium. As nickel is present, the fluorescence intensity of the solution decreases. Results The wavelength of excitation was 492 nm and the wavelength of emission was 516 nm. The reaction was optimized at 100 ℃ for 10 min. The linear range of determination was 4.0-80.0 ?g/L and the detection limit was 2.2 ?g/L . The RSDs and recovery rates of the method were 2.6%-3.2%, and 96.7%-102.8% respectively. Conclusion This method is simple, rapid and sensitive for the determination of trace nickel in human hair samples.