Hyperoside attenuated hypoxia-induced memory impairment by antioxidative activity / 局解手术学杂志

Objective To determine the effects of Hyperoside ( Hyp) on the memory impairment induced by hypobaric hypoxia. Meth-ods SD rats were exposed in hypobaric hypoxia chamber which simulated 6 100 m high altitude for 7 days to induce memory impairment. Morris maze was applied to determine the effects of Hyp on memory in hypobaric chamber. The activities of SOD and CAT and levels of GSH and MDA in rat hippocampus were measured by assay kit ( Nanjing Jiancheng Bioengineering Institute) . Results In target quadrant,the time and path and the frequency to cross platform were reduced under hypobaric hypoxia as compared with normoxia group ( P < 0. 05 or P<0. 01). Administration of Hyp significantly attenuated the hypoxia-induced the memory impairment responses(P<0. 05 or P<0. 01). The activities of SOD and CAT in rats hippocampus exposed to hypobaric hypoxia were significantly improved in the presence of Hyp as com-pared with the absence of Hyp(P<0. 05 or P<0. 01),so did GSH level (P<0. 05 or P<0. 01). Significant decrease of MDA levels was observed in the hippocampus on exposure to hypobaric hypoxia with Hyp as compared without Hyp. Conclusion Hyp reduces hypoxia-in-duced oxidative stress injury by upregulating activity of antioxidant proteins such as SOD,CAT in hippocampus of rats, which contribute to at-tenuate memory impairment induced by hypobaric hypoxia.

The way the local colleges and universities strengthen discipline construction / 中华医学教育探索杂志

This article has illuminated the importance of strengthening discipline construction to the the local colleges and universities,and why Guangdong Pharmaceutical University should take all kinds of measures to serve the development of territorial economy and improve the characteristic for operating the universities.

Effects of different therapeutic methods and typical recipes on activation of ERK1/2 in Kupffer cells of rats with fatty liver / 中国病理生理杂志

AIM: To investigate effects of different therapeutic methods and typical recipes on activation of ERK1/2 in Kupffer cells of rats with fatty liver.METHODS: The rat model of fatty liver was established by feeding high fat diet combinated with distillate spirit.Meanwhile Chinese medicines Shugan fang,Jianpi fang,Huoxue fang,Qushi fang,and Zonghe fang were given to treat different groups respectively.12 weeks later,the Kupffer cells were isolated from livers of control group,model group and different treatment groups by sequential in situ perfusion with collagenaseⅣ and pronase E,density gradient centrifugation,selective adherence.The expression of total ERK1/2 and phospho-ERK1/2 in Kupffer cells of control group,model group and different treatment groups were detected by Western blotting.RESULTS: The expressions of total ERK1/2 and phospho-ERK1/2 were higher in Kupffer cells from model group than those in control group(P