Effects of training programs of pregnant women on their delivery type selection: a single blind randomized control trial

There has been an increasing demand for cesarean section delivery [C/S] by pregnant women in recent years. This increase in demand, not only adversely affects the rate of delivery complications, but also imposes a great amount of extra expenses on families, government and insurance companies. It was assumed that by training programs for pregnant women during their prenatal care visits, they can play an active role in the process of decision-making regarding their type of delivery. The goal of the present study is to determine the effect of such training programs on pregnant women in selecting the type of the delivery and to specify if the current rate of C/S can be change by such training programs. In this single-blind randomized control trial, 200 primi-par pregnant women [age 18-30 years old] with gestational ages of less than 30 weeks, referring to the Ob-Gyn clinic of Be'sat general Hospital [IRIAF central hospital] for prenatal care were included.These pregnant women were randomly divided into two groups: Group A which received planned training program during their pregnancy and group B with no training program. None of the assessors knew about the women's status in the mentioned groups. Distribution of women in both groups were equal considering factors like age, education, profession and selected method of delivery at their first interview. In group A, films and pamphlets were used for training. Pamphlets were explained by professional midwives. Patients visited the Ob-Gyn department and delivery ward and spoke to women in their post-delivery phase. At their 36 week of gestational age, all patients in both groups were interviewed again and data were collected and analyzed by proper software using chi square test. In group A, the level of patient's awareness regarding Normal Vaginal Delivery [NVD] and cesarean section [C/S] was markedly increased, expectedly [78%vs.4%] and [71% vs. 4%], while in group B the level of patient's awareness did not changed on their second interview. The rate of selecting C/S in group A [test] was markedly reduced [25%vs. 39%], [p < 0.05], while there were no changes in this rate among patients in group B [40% vs. 41%]. can be concluded that basic trainings for pregnant women can effectively reduce the rate of demand for C/S delivery. It is therefore, recommended that this kind of training to be available for all patient [regardless of their level of education] referring to prenatal care clinics

Alginate extraction of Pseudomonas aeroginosa and study of penetration of antibiotic agents through alginate using a sandwich cup method

Pseudomonas aeroginosa is one of the most important opportunistic bacteria which produces biofilm. It has been reported that biofilm forming bacteria are more resistant to antibiotic treatment and immunologic response. Biofilms, communities of cells adhering to a surface enclosed in a self-producing polymeric matrix. Biofilms might be responsible for 65%of bacterial infections. In this study we investigated the role of the barrier formed by alginate against antibiotic penetration .Mucoid isolates of P. aeruginosa were collected from 2 hospitals and identified by biochemical tests. Strain M214 P. aeruginosa produces more biofilm compared with other strains. Alginate was extracted from mucoid type P. aeruginosa [M214] with method described by Goven. We evaluated the penetration of antibiotics macrolides [azithromycin,erythromycin],aminoglycosides [amikacin,gentamicin] lactames [imipenem,ceftazidem],fluoruqinolones [ciprofloxacin,ofloxacin] through P. aeruginosa [M214] alginate with a sandwich cup method described by Hiromi Kumon indicated that macrolides demonstrated penetration rate 100% .fluroquinolones and lactames demonstrated relatively high penetration rate>85%, whereas aminoglycosides showed low penetration [amikacin=59%,gentamicin=73%]. In this study, the role of alginate as a barrier against antibiotics penetration was proven