Leucocytes show improvement growth on PHA polymer surface

Polyhydroxyalkanoate [PHA] from one fermentation process shows diverse physical properties when extracted using different methods. Pseudomonas aeruginosa strain has been previously isolated from the Egyptian ecosystem was cultivated on olive oil as a carbon source under PHA accumulation conditions. PHA was extracted using four different extraction methods and the polymer give different biological properties. Leucocytes grown in different rate on each preparation. RBCs haemolysis test was used to determine the polymers toxicity. PHA isolated directly with chloroform give the highest leucocytes number [19.4 10 4 Cells/48 hr] and the lowest Haemolytic index [2.28]. Bioassays used in this study are recommended for evaluating the in vitro polymer biocompatibility aiming to in vivo application or as a cell line-supporting matrix

Role of CT virtual colonoscopy in colonic neoplastic lesions in comparison with conventional colonoscopy

Cancer colon is one of the commonest neoplasms all over the world and is considered a leading cause of death among different neoplasms. Virtual Colonoscopy is one of the recent advances in the field of mutidetector CT post processing technique. It represents a type of 3D reconstruction technique which is capable of elaborating endoscopic endoluminal display of the colon without use of real endoscope and hence it was named virtual colonoscopy. The aim of work is to evaluate the role of virtual colonoscopy in colonic neoplasia. Results were compared to conventional colonoscopy and documented by histopathology in all cases. Our study included 35 patients; 25 of them had positive colonic neoplasia. They ranged in age from 22 to 79 years. The results in our study showed that conventional colonoscopy is relatively of virtual colonoscopy, yet the results are more or less comparable to each other. We discussed the advantages and limitations of conventional and virtual colonoscopy