Construction of azospirillum lipoferum gene Library and cloning of Trans-zeatin secretion [TZS] gene

A gene library of total DNA of A. lipoferum ATCC 29708 was constructed using the cosmid vector pV 34. More than two thousand transductants were isolated with inserted fragments of 36.45 kb in average. Trans- zeatin secretion [tzs] gene was isolated by screening the gene library by DNA hybridization using the tzs gene of Agrobacterium tumefaciens as a probe. Three clones were found, p MAZ 1, 2 and 3. The clone p MAZ 1 was 43.5 kb and the tzs gene was localized on the eight kb-Sal I-fragment or on the 5.2 kb fragment of the double digestion Eco RI/Sal I of this plasmid. The successful cloning of tzs gene of A. lipoferum is an evidence for the involvement of DMA transferase enzyme in trans-zeatin [tz] production in Azospirillum