RESUMO
Background: Polycystic ovary syndrome [PCOS] is an endocrine disorder featured by insulin resistance and hyperandrogenism. Testosterone enanthate can induce PCOS in mice models
Objective: We investigated the ovary stereological features along with the oxidative stress and inflammatory factors in mice following PCOS induction using testosterone enanthate
Materials and Methods: Twelve female NMRI mice [3 wk old] were divided into 2 groups [n=6/each]: Control and PCOS. PCOS was induced through daily injections of testosterone enanthate [1 mg/100g subcutaneous s.c for 5 wk]. Finally, ovaries were studied stereologically. The serum levels of the follicle-stimulating hormone, luteinizing hormone, testosterone, interleukin-6, and tumor necrosis factor-alpha were measured using ELISA kit. Serum levels of Malondialdehyde and the antioxidant capacity were measured relatively using thiobarbituric acid and ferric reducing antioxidant power assay
Results: The mean total volume of ovary and the mean volume of cortex [p<0.001], volume of oocyte in the preantral [p=0.011] and antral follicle [p=0.015], thickness of zona pellucida [p=0.016], the number of antral follicles [p=0.012], the serum levels of follicle-stimulating hormone [p<0.001] and the antioxidant capacity [p=0.020] reduced significantly in the PCOS group compared to the control. The number of primary [p=0.017] and preantral [p=0.006] follicles and the serum levels of testosterone [p<0.001], Luteinizing hormone [p=0.002], Malondialdehyde, Interleukin 6 and Tumor necrosis factor-? [p<0.001] showed a significant increase in the PCOS group compared to the control
Conclusion: Testosterone enanthate induced PCOS causes stereological features in the ovary, increases the oxidative stress and inflammatory markers in mice