Vinegar as a removing agent of candida albicans from acrylic resin plates

Adherence of Candida species, mainly C. albicans to denture surfaces, forms a biofilm which causes denture stomatitis in denture users. Removal of Candida plaque on dentures is essential to control the colonization of this yeast and to prevent infections related to C. albicans. The aim of this study was to compare the effectiveness of sodium hypochlorite with white vinegar for the disinfection of C. albicans from acrylic resin. 82, 10x10x1 mm acrylic resin plates were inoculated with 1x103 C. albicans suspension for 24 hours to prepare experimental Candida biofilm. The total number of Candida cells which adhered to 10 acryl resin plates was determined and the remaining 72 plates were randomly divided into four groups. The test plates were immersed in a solution of 1% sodium hypochlorite, 5% or 10%white vinegar for a period of 8 hours and distilled water was used as the negative control group. The Candida removing ability of the 3 disinfectants and the negative control group was assessed by comparing the number of colony forming units per 1 mL of the plates washing solution before and after the removing protocol. Data was analyzed using Kruskal-Wallis and Mann-Whitney tests. Sodium hypochlorite [1%] and white vinegar [10%] removed 100% of the C. albicans cells, followed by white vinegar [5%], which removed 99% of the adhered C. albicans from the acrylic resin plates. There wasn't any significant statistical difference found between the 5% and 10% white vinegar in removing Candida from the acryl resin plates [P = 0.161]. Overnight immersion of complete removable dentures in 10% or even a 5% white vinegar solution effectively removed C. albicans cells that had adhered to the denture surface and their removal properties the same as 1% sodium hypochlorite.

[ survey of microbial contamination of new acrylic removable denture's made in Yazd [Iran] dental laboratories 2009]

Microbial contamination of removable denture and their contact with saliva and oral tissues can cause microbial cross-contamination among dental clinic personnel, dental laboratories and patients. These microorganisms can cause unpredictable infections especially systemic, pneumonia and even cardiac infections in elderly and immunosupressed patients. The aim of this study was to identify the microorganisms on the surface of removable prostheses ready for delivery [new dentures] in Yazd dental laboratories Fifty five new removable complete dentures were randomly selected from 5 Yazd dental laboratories. All dentures were washed by 100ml of sterile normal saline, centrifuged and precipitants were used for culture on the specific microbial media. Finally, the isolated aerobic, anaerobic microbial colonies and isolated fungi were identified according to diagnostic tests. Bacterial contaminations were seen in all prostheses whereas 58.2% showed fungal contaminations. Staphylococcus, nonpathogenic Neisseria spp, Corynebacteria, Acenitobacteria, E. coli, and Entrobacter spp were the common isolated bacteria in the current study. Saprophyte fungi such as Aspergillus, penicillium, mucor and yeasts especially Candida species were the most isolated fungi from dental prostheses in the present study. Results of the present study showed bacterial and fungal contamination even on newly made prostheses. It seems that control and prevention of cross-contamination should be taken more seriously in dental practices and laboratories

comparison of the efficacy of nystatin and fluconazole incorporated into tissue conditioner on the in vitro attachment and colonization of candida albicans

Denture stomatitis is a common oral lesion following the use of ill-fitting dentures. A layer of tissue conditioner is usually used to improve adaptation of the denture. These liners can support the in vivo adhesion and colonization of the oral Candida. The aim of this study was to evaluate the efficacy of the two common antifungal agents mixed with tissue conditioner against Candida al bicans. Tissue conditioner disks [Acrosoft] with 5mm diameter and 1mm thickness containing different concentrations of nystatin and fluconazole [1%, 3%, 5%, 10% wt/wt] as well as disks with no antifungal agents [8 disks for each group] were prepared for experimental biofilm formation by inoculation with Candida albicans cell suspensions. The specimens were incubated in cell culture microtiter plate wells containing Sabouraud's broth in a rotator shaker at 30°C for 48 hours. Then, the specimens were rinsed and sonicated in sterile water to remove surface organisms. The attached yeasts were enumerated by inoculation of the yeast suspension on Sabouraud's agar. The data was compared using Kruskal-Wallis and Dunn's tests using prism software. P value less than 0.05 was considered significant. The 1% to 10% mixture of nystatin and tissue conditioner completely inhibited the attachment and colonization of Candida albicans, although for fluconazole only a 10% concentration caused complete inhibition. Nystatin showed a potentially higher effect in inhibition of Candida attachment and colonization [P = 0.0001] compared to that of fluconazole and a statistically significant difference was seen between 5% and 1% fluconazole [P = 0.0001]. Tissue conditioner with 1% to 10% nystatin or 10% fluconazole can completely inhibit the adhesion and colonization of Candida albicans

Candida species colonization level in elderly angular cheilitis patients before and after denture replacement

A decrease in the vertical dimension of occlusion in elderly patients using very old dentures promotes colonization of oral Candida, resulting in angular cheilitis. Correction of the vertical dimension of occlusion by replacement of the dentures can reduce colonization of Candida and improve angular cheilitis lesions. The aim of this study was to compare the colonization of Candida in the angular cheilitis lesions of elderly before and after denture replacement to demonstrate the role of modification in vertical dimensions of occlusion on oral hygiene in the elderly. Twenty-four patients with complete dentures and angular cheilitis, who presented for denture replacement, participated in this study. Samples were taken from their lips and cultured before and 3 months after replacing their denture. The mean density of isolated colonies before and 3 months after denture replacement were compared using the Wilcoxon rank test. All samples were heavily colonized before denture replacement, though 3 months after using new dentures only few cultures were found to be positive. A statistical significant difference was obtained [Wileoxon rank test] between colonization of Candida species before and 3 months after denture replacement [P = 0.0001]. Results revealed that using dentures for long period of time may cause a decrease in vertical height This phenomenon caused a wide range in colonization of oral Candida species, which directly resulted in angular cheilitis, This could be prevented by using new dentures to improve oral health