RESUMO
Background: Troxerutin is a flavonoid antioxidant that protect different organ against damage caused by ischemia-reperfusion
Objective: The aim of this study was to evaluate the effect of troxerutin in reducing the damages caused by ischemia-reperfusion in rat's testis
Materials and Methods: 40 Male Wistar rats [2 month old] were divide to four groups [n=10]. Group1 [sham], Group 2 [control, ischemia-reperfusion [I/R] without treatment], Group 3 [I/R+150 mg/kg of troxerutin], and group 4 [I/R+20 mg/kg of vitamin C]. Treatment of group 3 and group 4 during torsion [twists 720 counter clock at 90 min] followed by 50 days detorsion. After 50 days, blood samples were collected and rats in all study groups were killed and their testes were removed, and fixed with Bouin's solution. Testis was stained with hematoxylin and eosin dye and the level of testosterone, luteinizing hormone [LH] and follicle-stimulating hormone [FSH] were measured with ELISEA methods. TUNEL was employed to detect apoptosis. Epididymis caudal part was removed and total sperm count was determined. Johnson techniques were used for assessment of seminiferous tubules quality
Results: Troxerutin treated group has higher Johnson scorefs [p
Conclusion: Troxerutin has protective effects on testicular torsion induced injury and can ameliorate spermatogenesis in the torsion-detorsion models
RESUMO
Spermatocytogenesis starts from lumens of seminiferous cords and after migration to the basal membrane ends to the lumens again. We attempt to review the protective effects of melatonin and ghrelin on Spermatocytogenesis and in particular on spermatogonial stem cells, as two rather newly-discovered hormones. Testicular freezing prior to chemotherapy and radiotherapy is one of the ways of preserving fertility in children with cancer. The freezing has two methods of slow-freezing [cryopreservation] and rapid-freezing [vitrification]. Administration of melatonin can maintain the quality of the germ cells underwent such processes, as well as ghrelin, can protect germ cells from the toxicities secondary to ischemic injuries, and pathologic apoptosis. This review indicates that in vitro or in vivo administration of melatonin or ghrelin, could be effective to preserve fertilization and also they can be used in assisted reproductive technologies to improve the quality of sperms. Future original studies should be propelled toward human studies, of course with observing the ethics
RESUMO
Aim: The aim of this study is to demonstrate the relation between the expression of liver alpha-amylase and obesity
Background: Alpha-amylase catalyses the hydrolysis of 1, 4-alpha-glucosidic linkages in polysaccharides and has three main subtypes, including: salivary, pancreatic, and hepatic. Hepatic alpha-amylase is involved in glycogen metabolism, and has a role in obesity and its management. In this study, we aimed to analyze the expression of liver alpha-amylase in overweight and obese mouse
Material and methods: In this study, NMRI male mice were randomly divided into two groups. The sample group [obese] took a high-fat and carbohydrate diet, while the control group [normal] took a laboratory pellet chow for eight weeks. During this period, their weight was measured. After eight weeks, liver hepatocytes were isolated using an enzymatic digestion method. Immunocytochemistry [ICC] and flow cytometry analysis were performed to measure alpha amylase protein expression in mouse liver hepatocyte cells
Results: A significant difference in the body weight was observed between the two groups [p<0.05]. The qualitative protein expression of liver alpha-amylase was found to be higher in the obese group in both tests [immunocytochemistry and flow cytometry]. Animals from the test group presented higher alpha-amylase expression, which suggests that this hepatic protein may constitute a potential indicator of susceptibility for fat tissue accumulation and obesity. The present data demonstrates an increased expression of liver amylase in obese mice
Conclusion: These results suggest that liver amylase secretion might be useful for predicting susceptibility to obesity induced by consumption of a high-fat and carbohydrate diet