RESUMO
Objective: Forkhead box [FOX] proteins are important regulators of the epithelial-to-mesenchymal transition [EMT], which is the main mechanism of cancer metastasis. Different studies have shown their potential involvement in progression of cancer in different tissues such as breast, ovary and colorectum. In this study, we aimed to analyze the expression of genes encoding two FOX proteins in gastric adenocarcinoma
Materials and Methods: In this experimental case-control study, the expression of FOXC2 and FOXQ1 was examined in 31 gastric adenocarcinoma tumors and 31 normal adjacent gastric tissues by reverse transcription polymerase chain reaction [PCR]
Results: The expression of both genes was significantly up-regulated in gastric adenocarcinoma tumors compared with the normal tissues [P<0.05]. The differential expression of these two genes was also correlated with the grade of tumors [P<0.01]
Conclusion: We show that up-regulation of FOXC2 and FOXQ1 are likely to be involved in the progression of gastric adenocarcinoma
Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Fatores de Transcrição Forkhead , Regulação para Cima , Adenocarcinoma , Expressão Gênica , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Estudos de Casos e ControlesRESUMO
HOTAIR is an lncRNA that has been known to have an oncogenic role in different cancers. There is limited knowledge of genetic and epigenetic elements and their interactions for the gene encoding HOTAIR. Therefore, understanding the molecular mechanism and its regulation remains to be challenging. We used different in silico analyses to find genetic and epigenetic elements of HOTAIR gene to gain insight into its regulation. We reported different regulatory elements including canonical promoters, transcription start sites, CpGIs as well as epigenetic marks that are potentially involved in the regulation of HOTAIR gene expression. We identified repeat sequences and single nucleotide polymorphisms that are located within or next to the CpGIs of HOTAIR. Our analyses may help to find potential interactions between genetic and epigenetic elements of HOTAIR gene in the human tissues and show opportunities and limitations for researches on HOTAIR gene in future studies.
Assuntos
Humanos , Biologia Computacional , Simulação por Computador , Ilhas de CpG , Epigenômica , Expressão Gênica , Polimorfismo de Nucleotídeo Único , RNA Longo não Codificante , Sítio de Iniciação de TranscriçãoRESUMO
Different mechanisms, including transcriptional and post transcriptional processes, regulate tissue specific expression of genes. In this study, we report differences in gene/protein compositional features between apoptosis involved genes selectively expressed in human tissues. We found some correlations between codon/amino acid usage and tissue specific expression level of genes. The findings can be significant for understanding the translational selection on these features. The selection may play an important role in the differentiation of human tissues and can be considered for future studies in diagnosis of some diseases such as cancer.