Pathobiochemical studies on the effect of cytoxan, 5- fluorouracil and methotrexate [CMF] on mice intestine

The effect of cyclophosphamide, Methotrexate and Fluorouracil [CMF] was investigated on intestinal enzymes [5-nucleotidase, Alkaline phosphatase and Glucose-6-phosphatase] as well as on the histopathology of intestinal mucosa of mice. The drugs were given either as single agent or in combination in two dose schedule, a one day puls therapy or once every other day for 5 doses. Intestinal 5-nucleotidase is the most significantly elevated enzyme and no sign of recovery was observed 10 days after [CMF] administration. Histopathology of mice intestine showed that the initial changes that were observed by repeated small doses were more severe than those observed after single pulse administration, recovery of which needs more than ten days