RESUMO
A novel approach has been described. In this method the serum IgG level can be predicted with a fair degree of accuracy by scanning densitometry and total serum protein values. This approach might be useful as a screening procedure for hypogammaglobulinemia.
Assuntos
Agamaglobulinemia/diagnóstico , Eletroforese das Proteínas Sanguíneas , Proteínas Sanguíneas/análise , Densitometria , Eletroforese em Acetato de Celulose , Humanos , Imunoglobulina A/análise , Imunoglobulina G/análise , Imunoglobulina M/análiseRESUMO
Most existing methods for detecting antigiardia lamblia antibody require the use of cultured Giardia lamblia trophozoites as antigen in immunofluorescent and enzyme-linked immunosorbent assays. However, the maintenance of trophozoite culture systems limits the large-scale use of these antibody detection systems. An antigen extracted from Giardia lamblia by the detergent Triton X-100, when used in an ELISA system, produces specific, objective, quantitative and reproducible results. It is likely that such a test system could be packaged in kit form for large-scale diagnostic and epidemiological application in all parts of the world.