Assessment of genetic diversity in some Iranian populations of Bunium persicum using RAPD and AFLP markers

The genetic diversity of 20 Iranian populations of Bunium persicum has been evaluated with random amplified polymorphic DNA [RAPD] and amplified fragment length polymorphism [AFLP] markers. Fresh leaves of seedlings from each population were used for genomic DNA extraction. Analysis of banding patterns of 15 RAPD primers and 17 AFLP primer combinations, revealed 192 [86%] and 228 [75%] polymorphic bands, respectively. The range of similarity coefficients within populations were 0.4-0.82 for RAPD and 0.39-0.96 for AFLP markers. No association was observed between similarity matrices of both the DNA markers. Genetic distance patterns between B. persicum populations, expressed by the RAPD and AFLP cluster analyses were relatively different. The resulting dendrograms based on AFLP and RAPD +AFLP markers were more similar when compared to that derived from RAPD analysis. The AFLP generated dendrogram was supported with the highest bootstrap values. The measures of relative genetic distances among populations did not completely correlate with geographical distances of places of their origin. Several populations of black cummin were represented as independent groups in the clusters, showing a high level of genetic diversity and unique genetic background. Knowledge of wide genetic diversity observed in the B. persicum populations provides important information for management of germplasm resources with regard to future domestication and breeding programs

Study of genetic variation in wild diploid wheat [triticum boeoticum] from Iran using AFLP markers

Little information is available regarding genetic variation in wild wheat relatives from Iran. In this study, genetic diversity of 36 populations of wild einkorn wheat, Triticum boeoticum, was studied using amplified fragment length polymorphism [AFLP] primer combinations. Seventeen AFLP primer combinations led to amplify 979 scorable fragments ranging from 50 to 500 bp and of these, 429 [44%] were polymorphic across the 36 populations. The average Dice genetic similarity between T. boeoticum populations was 0.67 [range= 0.18-0.98]. The dendrogram derived by unweighted pair group method with arithmetic mean algorithm [UPGMA] analysis revealed three main groups. PCO analysis also confirmed subgrouping obtained by cluster analysis. The measured relative genetic distances among accessions was not correlated with geographical distances of places of their origins, indicating that the populations are genetically different. The results demonstrated that AFLP technology is a suitable technique for genetic resource management in T. boeoticum populations of these studied origin sites

Analysis of the genetic diversity 12 Iranian Damask rose[Rosa damascena mill.] genotypes using amplified fragment length polymorphism markers

In this study, the genetic diversity of 12 Iranian Damask rose [Rosa damascena Mill.] genotypes was studied using amplified fragment length polymorphism [AFLP] markers. Twelve AFLP primer combinations generated 483 polymorphic bands and showed extreme variability and genetic complexity among the studied genotypes. The AFLP analysis revealed a specific amplified fragment for the genotypes collected from the Meymand region. Despite the geographical distance between Kashan and Kazeroon regions, the results of cluster analysis showed that the genotypes collected from these districts regions were genetically related. The low genetic similarities were observed among the genotypes belonging to Tabriz, Meymand and Kashan regions suggesting that there was relatively less geographical relocation of the genotypes between these regions. Hence, for breeding of the Damask roses taken in this study, it is necessary to conjunct the molecular data with agronomic characters