Changes of pH produced by immersion of endodontic sealers

When evaluating the antimicrobial effect of endodontic sealers it is important to study the variation of pH, since this is one of the properties that may inhibit the microbial growth and influence biocompatibility. The aim of this study was to evaluate the capacity of several endodontic sealers without calcium hydroxide to modify the pH of the solution in which they were immersed. The sealers used were: Endomethasone C-Septodont (EC), Bio Seal-Ogna (BS), Diaket-Espe (D), Procosol-StarDental (P), AH26-Dentsply (AH), Ketac Endo-Espe (KE), AHPlus-Dentsply (AHP), Endion-Voco (E), Endomethasone-Septodont (EM), EZ Fill-Essential Dental Systems (EZ). Samples of each material were placed in tubes with phosphate saline solution (PBS). The pH was determined at 2, 5, 12, 20, 30, 60, and 90 days. A tube with solution alone was taken as control. Statistical analysis of the results was performed by ANOVA. Material, sample, time and material-time interaction elicited statistically significant differences (p < 0.05). The variation of pH produced by immersion of endodontic sealers depends on the composition of the material.

Antimicrobial power of composite resin adhesion systems

The adherence of microorganisms to dentin that had been contaminated and then treated with conditioning substances was evaluated. The germicide effect of those products and their possible substantivity was also evaluated. Dentin slices were contaminated with Candida albicans, Streptococcus mutans and Actinomyces naeslundii and then treated with the following substances: 1. Experimental Blue Solution A, 2. Experimental Red Solution B; 3 Tubulicid Blue Label; 4. Tubulicid Red Label; 5 Scotchprep Dentin Primer; 6 ventura Dentin Bond Cleaner; 7 ventura Dentin Bond Primer, 8. Gluma dentin Bond; 9 Tenure Conditioner; 10. All Bond Dentin Conditioner; 11 Syntac Primer; 12. Clearfil New Bond acid + adhesive; 13 Prisma Universal Bond 3 Primer; 14. Denthesive Cleaner; 15. Control (Distilled water). Adherence was evaluated using scanning electron microscopy and viability tests were performed. Substances 1, 2, 3, 4, 5, 7, 8, and 11 impair adherence; the control and substances 6 and 9 produced no afforded intermediate results effect while substances 10, 12, 13 and 14. Viability tests afforded results similar to those obtained for adherence. Several substances that are used for dentin treatment or for dentin priming for adhesion could be useful in preventing microorganism viability and so contribute to the protection of pulp vitality.

Bacterial inhibition produced by substances for dentin pretreatment

Dentin treatment before adhesion of composites is performed both to enhance adhesion and to remove the microbial contents of the smear layer. The purpose of these experiments was to evaluate the germicide potential of several dentin treatments used in adhesive systems and of some cleansing solutions. Different germs involved in caries processes were used (Candida Albicans, Streptococcus mutans and Actinomyces naeslundii) to prepare suspensions. Half a milliliter of each of the suspensions was transferred to test tubes and an equal volume of the following substances was added: Scotch Prep Dentin Primer (P), Gluma Cleanser (G), Cleaner Sol. (C), Tubulicid Blue (TB) and Red Label (TR), Blue Experimental Solution (SB) and Red Experimental Solution (SR) and sterile distilled water (control). The preparation was incubated at 37 degrees C for seven days to test viability. P, TR, TB and SB produced complete inhibition of germs tested. The results reveal that, [quot ]in vitro[quot ], not all the substances tested exert a germicide effect on the microorganisms analyzed.

Adherencia de candida modificada por la acción de antisépticos / Adherence of candida albicans modifiqued for the action the antisepsic

La capacidad de los microorganismos para adherirse tiene importancia en el mecanismo de la formación de placa "in vitro". El objetivo de este trabajo fue investigar la posible acción antiadherente de antisépticos, de uso oral; la influencia ejercida por la presencia de sacarosa y las modificaciones que se producirían si se asociara un microorganismo de la flora indígena bucal dominante con otro de la flora accesoria. Se prepararon suspensiones homologadas con el n§3 de la escala de Mac Farland de 10 cepas de Candida albicans y una suspensión de Streptococcus sanguis. Cada suspensión de Candida albicans fue distribuida en 12 tubos a razón de 0,5 ml en 10 de ellos y 0,25 en los tubos restantes; a estos se le completó el volumen con 0,25 ml de la suspensión de Streptococcus sanguis. En cada tubo agregamos 0,5 ml de los distintos antisépticos en estudio (Fluoruro de sodio (NaF) en distintas concentraciones, clorhexidina al 0,2 % floruro estañoso, povidona y agua destilada estéril (testigo)). El conjunto fue incubado 2 hs. en agitación constante a 37§C. Se lavaron las células una vez y se restituyó el volumen. 0,5 ml de cada uno se sembró en tubos que contenían 5 ml de medio base para la formación de placa "in vitro" con y sin el agregado de sacarosa. De los tapones pendían trocitos de diente estéril. Se incubó 5 días a 37§C. Transcurrido este tiempo procedimos a interpretar los resultados por el aspecto macroscópico de los cultivos y se procedió a la fijación de los dientes para su posterior observación por Microscopía electrónica de barrido (M.E.B.). Algunos de los antisépticos ensayados, ejercen una débil acción candicida, no se demostró influencia en la adherencia. La presencia de sacarosa en el medio es un factor importante para la formación de placa "in vitro"; la asociación de Candida albicans y Esteptococcus sanguis no influye en la misma

Evaluacion de aspectos de la inmunidad celular en drogadictos. / Evaluation of the cellular immunity aspects in drug dependents

Se evaluo la inmunidad celular en 52 enfermos dragadictos, comparandola con un grupo de 30 no adictos. Para tal fin se utilizaron pruebas cutaneas con derivado proteinico purificado, candidina, parche de dinitroclorobenceno, prueba de la inhibibion de la migracion de linfocitos con derivado proteinico purificado de tuberculina y candidina, y ventana cutanea. Los resultados fueron diferentes en ambos grupos e indicarian que los toxicomanos tenian deprimida la respuesta celular