Role of bone marrow transplantation in reducing the dangerous effect of multi-walled carbon nanotubes or/and gamma rays on male rats

Aim of the work: this study evaluated the efficacy of bone marrow [BM] transplantation to protect male rats from dangerous effect of multi-walled carbon nanotubes [MWCNTs] or/and y-irradiation on the hematopoietic and lung tissues

Materials and methods: experimental animals were divided into 8 groups each consist of 6 male albino rats. Control group, BM-injected group, MWCNTs-injected group, 5Gy y-irradiated group, 5Gy y-irradiated+ MWCNTs-injected group, MWCNTs-injected+ BM-injected group, 5Gy y-irradiated+ BM-injected group and 5Gy of y-irradiated+ MWCNTs-injected + BM-injected group. All the treated animal groups were sacrificed after 28 days of the treatments. Blood components, MDA and GSH levels in the lung tissue were analyzed. The histopathological study in the lung tissue was also recorded

Results: exposure to MWCNTs or/and y-radiation induced a significant decrease in certain blood components [WBCs, RBCs, Hb content, HCT value and PLT count]

Furthermore, a significant elevation in MDA level and a significant decrease in GSH content were detected in the lung tissue

The histopathological changes after exposure of rats to MWCNTs recorded perivasculitis, atelectasis and interstitial pneumonia. Also, y-radiation represented more collapsed and thickened walls in the alveoli, thickened bronchiolar walls with partial epithelial lining and foci of pulmonary hemorrhage in the lung tissue. The effect of BM transplantation after MWCNTs or/and y-radiation ameliorated the values of blood components, MDA and GSH levels in the lung tissue. The improvement occurred by BM transplantation in rats treated with MWCNTs or/and exposed to y-radiation were also recorded. The lung tissue showed numerous alveoli with thin interalveolar septa, alveolar sacs and terminal bronchioles with highly folded mucosa

Conclusion: treatment with BM transplantation improved the most deleterious parameters obtained in the blood and lung tissue of MWCNTs exposed or/and y-irradiated rats

Biological studies on fresh water snails target to Schistosoma mansoni infection

In this study ,upon exposure of laboratory Egyptian B. alexandrina and B. glabrata snails to its homologus strain of S. mansoni, two different phenotype strains " susceptible and resistant " were distinguished . The aim of this study is to investigate the biological differences between the two types of Biomphalaria snails either susceptible or resistant at different ages. The investigated parameters included mortality, survival, percentage of susceptibility / resistance to parasitic infection and fecundity. The results of the preliminary exposure experiment revealed that parental snail stock was not completely susceptible and tend to be partially refractory to infection. The results also showed a strong association between the levels of egg production and susceptibility to parasite infection. Where breeding experiment revealed that, total egg number, E/M and EM values produced by resistant phenotype of both snail species were more than those produced by susceptible progeny snails. Also, it was noticed that, egg production is associated inversely proportion with snail age