Effect of concommitant Helicobacter pylori infection in patients with Giardiasis lamblia in Egypt

Giardiasis is one of the most common enteroprotozoal diseases; its association with Helicobacter pylori is a common clinical finding. This work studied the impact of such association. Fifty giardiasis patients were classified into two groups according to the concomitant presence of H. pylori and ten normal healthy controls were also included. All patients were subjected to complete history taking, thorough clinical and stool examination, endoscopy, and biopsy of gastric and duodenal mucosa as well as histopatological examination. Results revealed significant upper gastrointestinal symptoms [epigastric pain and anorexia] in giardiasis patients with H. pylori. Also, endoscopic and histopathologic examination showed significant gastric lesions in this group of patients as compared to those suffering only G. lamblia

Impact of helicobacter pylori infection on the activities of urease and lipase enzymes in patients with giardiasis

The study of the effect of Giardia lamblia and Helicobacter pylori organisms coexistence on the activities of urease and lipase enzymes was the aim of this work which was done through choosing 50 cases of giardiasis in addition to 10 normal individuals chosen as a control group [free from giardiasis]. It is well known that H. pylori is considered one of the most important causes of gastric and duodenal inflammations which could predispose to ulcers and hypochlorhydria leading to increased susceptibility to giardiasis as it is known that HCl acts as a chemical barrier to microbes. The biochemical tests were done to investigate the activity of both urease and lipase enzymes extracted from the gastric juice of patients and controls. A significant increase in urease activity in the group having combined infection [giardiasis and H.pylori] than the group infected with G.lamblia alone and the control group was found. The same findings were obtained regarding the lipase activity. In the present work, both infections H. pylori and G. lamblia coexisted in 75% of epigastric pain cases which could be explained on the basis that both organisms predispose to each other

Protein enrichment of date waste by semi-solid fermentation

The semi-sold fermentation of date waste with Endomycopsis fibuligera can increase the concentration of the protein of the residue by up to 325 g/kg substrate. Optimal culture conditions were found to be 29 +/- 1° at pH 5.5 for 60 hr which produced a biomass yield 7.62 g/100g dry substrates. The starch content in the wet date waste culture was efficiently consumed [about 91%]. The bioconversion can be carried out to produce SCP with a high percentage of essential amino acids which increased its nutritional value to be used as animal feed. Total lipids and lipid component analyses revealed that double amounts found after 60 hr of fermentation

Biochemical effects of some insecticides on lysosomal enzymes in brain tissue

The effect of dimethoate, permethrin and AC 222-705, on the activity of brain beta-glucuronidase, acid ribonuclease and alpha- naphthyl acetate esterases of male mice was investigated. The data indicated that, these insecticides induce disturbances in the activities of the tested lysosomal enzymes, which constitute important indices for carcinogenicity

Production of ethanol by yeasts grown on hydrolysate of Egyptian sweet potato

The influence of different concentrations of HCl for acid hydrolysis of Egyptian sweet potato for maximal ethanol yield was studied. The optimum HCl concentration was 0.5 N with respect to reducing sugars formed [60% DE] and minimum hydroxymethyl furfural content [0.05%] at 97C for 20 minutes. Total solids for Egyptian sweet potato was 27%, reducing sugars and glucose content based on fresh weight were 27.53%, 26.7%, respectively. Both hydrolyzed sweet potato slurry [SPS] and glucose [at concentration 4.5%] were subjected to fermentation by 4 different yeast strains for 4 days of growth. The results showed optimum ethanol yield after 72 hours of fermentation with respect to Saccharomyces cerevisiae, Saccharomyces uvarum yielding 0.3 g/g, 0.31 g/g, respectively. Upon using Pachysolen tannophilus, the maximum ethanol yield was obtained after the first 24 hours 0.24% g/g and Pichia pinus was 0.27 g/g after 48% hours of fermentation. The yields of yeast cells were estimated after 96 hours of fermentation and indicated the presence of high dry weight of cells [4.0-8.5 g/L] with considerable amounts of crude protein [35-45%] and true protein [20.3-27.4%]

Lysosomal enzymatic activity in the sera and tissue of patients with thyroid disease

B-glucuronidase and total acid phosphatase enzyme activity was studied in sera and thyroid tissue of patients with toxic and malignant goitre. A highly significant increase in enzyme activity was observed. The significance of obtained results was discussed

effect of some insecticides on naphthyl acetate esterases activity of murine liver and spleen tissue homogenates

The oral administration of the pyrethroids insecticides AC-222, 705 and permethrin to the murine decrease the alpha-naphthyl acetate esterases enzyme activity of liver and spleen in both patches of single and repeated doses. This decreases in enzyme activity may be due to the hepatotoxicity from the cumulative effect of the insecticide and /or its metabolites. Moreover, the decreased activity may throw light on the mechanism leading to fatty changes in the liver of treated mice, since these enzymes are known to be concerned with lipid breakdown. The organophosphorus cyolane increased the activity of the enzyme in the liver homogenate which may be due to the inflammatory effect of the insecticide