RESUMO
The experiment was framed to get the most effective extract of Aloe vera (L.) leaf gel for the amelioration of hypo-functional activity of testis in diabetic model rats. Only one frequency of streptozotocin was injected through skeletal muscle at 40 mg/kg body weight for diabetes induction. Treatment with aqueous or ethanol or methanol or hydro-ethanol (40:60) or hydro-methanol (40:60) extract of Aloe vera (L.) was continued for 28 days. Rats were euthanized and sacrificed on 29th day. Fasting blood glucose level, kinetics of hexokinase, androgenic key enzymes, and markers of cellular oxidative stress were assessed. The concentration of the sperm per milliliter of epididymal washed fluid, sperm motility, serum testosterone, plasma insulin levels, lipid, and metabolic toxicity sensors were also measured. Significant amelioration (p ? 0.05) of the negatively deviated above-mentioned parameters and the disrupted histomorphology of testicles towards vehicle-treated control were noted after uninterrupted 28 days of treatment to diabetic rats with the mentioned extracts of Aloe vera (L.). The highest percentage of recovery in the adopted sensors was noted in the hydro-ethanol extract-treated diabetes group than others. Hydro-ethanol extract of the said plant part is potent among all other extracts for correcting such hypo-function of testicles in diabetes.
RESUMO
Traditionally Luffa acutangula (L.) Roxb. has been utilized as an herbal contraceptive. This in-vitro study has been performed to search out the male contraceptive efficacy of hydro-ethanol (60:40) extract of Luffa acutangula (LAHEE) seeds in a dose-specific manner (1, 2, and 4 mg/ml of in-vitro media). The percentages of motile, viable, hypo-osmotic swelled (HOS), and acrosomal intact of human and rat sperms were declined significantly (p<0.05) at the above-mentioned doses of LAHEE-exposed groups against the placebo group. The inhibitory concentration 50% value (IC50) of LAHEE was 2.5 mg/ml in human and 1.2 mg/ml in rat spermatozoa immobilization. The ?5,3? and 17? hydroxysteroid dehydrogenase (HSD) activities of rat’s testicular tissues were inhibited significantly at p<0.05 in LAHEE-treated groups than the placebo group. Activities of superoxide dismutase (SOD) and catalase were significantly inhibited (p<0.05) along with the significant increment (p<0.05) in the quantity of thiobarbituric acid reactive substances (TBARS) in rat’s testes and epididymis, sperm pellets of humans and rats in LAHEE-treated groups against the placebo group without any significant difference (p>0.05) in above said sensors in the liver and cardiac tissues of rats. The non-toxic nature of LAHEE was indicated by no significant alterations (p>0.05) in the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) after LAHEE exposure in said tissues of rats. The LC-MS study of LAHEE identified the presence of oleic acid, dihydroquercetin, luteolin-7-glucoside, formononetin, luteolin 8-C-pentoside-6-C-hexoside, pterosin B, boldine and berberine. These findings interpreted that the LAHEE possesses spermiological inhibitory and hypo-testicular activities, which indicate a promising possibility to develop a potent herbal male contraceptive agent from this plant extract.