RESUMO
Objective: To identify the anti-Toxoplasma antibodies from blood donors who referred to blood transfusion bases of Jahrom County, using ELISA method. Methods: Based on the prevalence and characteristics method, 400 serum samples were collected from blood donors referred to Jahrom blood transfusion bases, Southern Iran, during 2010-2011, designed at testing by ELISA. IgM and IgG antibodies against Toxoplasma gondii were tested using ELISA kits (Dia-Pro) on serums. The data were analysed by SPSS 19 software. Results: Review of 400 cases, 54 of them were IgG positive for parasites (13.5%) and 346 of those with negative IgG (86.5%). In IgM examination, 1.75% of them have been positive IgM (7 cases) and 98.25% of them were IgM negative (393 cases). By comparing the different group ages, 40-50 year age group had the highest prevalence of IgG positive (17.9%) and the age group of 30-40 years had the highest incidence of IgM negative (2.5%). Conclusions: Due to the serological infection rate of toxoplasmosis obtained from this study, toxoplasmosis should be considered as a significant transfusion risk factor in Jahrom and also in any region with similar situations.
RESUMO
Crimean-Congo hemorrhagic fever [CCHF] virus causes a severe hemorrhagic syndrome in humans with fatality rate up to 50%. Its transmission to humans is through the bite of Ixodid ticks or by contact with blood or tissues from infected livestock. By a nosocomial transmission of Crimean-Congo hemorrhagic fever [CCHF], a health care worker was infected in December 2008 due to a re-emerging outbreak of CCHF in Fars province, Iran. After admission of probable CCHF cases in a local hospital, one of the nurses contributed in taking care of the patients was infected with CCHF, though it seems that she had not had direct contact with blood and secretions of CCHF patients. The laboratory detected anti-CCHF virus IgM antibody through specific ELISA and also the CCHF virus genome in her serum by real-time and gel-based RT-PCR. She was improved by an alert and on time clinical diagnosis and treatment. We recommend that in outbreaks of CCHF, care to prevent airborne transmission should be kept in mind