RESUMO
INTRODUCTION: Breast cancer is one of the most common cancers in women; however, due to the complexity of chromosomal changes, limited data are available regarding chromosomal constitution. MATERIALS AND METHODS: In this study, Comparative Genomic Hybridization (CGH) was used on 16 Iranian patients diagnosed with invasive ductal breast carcinomas. RESULTS: 12 samples had abnormal CGH results (75%), including 21 types of chromosomal imbalance. The most prevalent were chromosomal gain of +1q, +17q, +8q and chromosomal loss of -13q. All three cases with DNA loss at chromosome 13q (-13q) had lymph node metastasis. CONCLUSIONS: CGH is able to detect chromosomal abnormalities which are difficult to identify by conventional cytogenetic techniques. More studies on a larger sample size may help to confirm or rule out any possible correlation between 13q monosomy and lymph node metastasis, which could result in establishing new strategies for prevention and early detection of invasive breast tumors.
Assuntos
Adulto , Idoso , Neoplasias da Mama/epidemiologia , Carcinoma Ductal de Mama/epidemiologia , Aberrações Cromossômicas , Cromossomos Humanos Par 1/genética , Cromossomos Humanos Par 13/genética , Cromossomos Humanos Par 17/genética , Cromossomos Humanos Par 8/genética , Hibridização Genômica Comparativa/métodos , DNA de Neoplasias/genética , Feminino , Humanos , Processamento de Imagem Assistida por Computador , Hibridização in Situ Fluorescente , Irã (Geográfico)/epidemiologia , Metástase Linfática , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , PrognósticoRESUMO
INTRODUCTION: As many as 30% of node-negative breast cancer patients relapse within five years, suggesting that current histological detection methods are inadequate for identifying metastatic disease. Detecting small number of cancer cells in the breast tissue or lymph node by reverse transcription-polymerase chain reaction (RT-PCR) assays using a combination of tissue and cancer specific markers might be very useful in the early detection or monitoring of the treatment. Mammaglobin is a member of the uteroglobin gene family and appears to be expressed only in breast tissue. Carcinoembryonic antigen has been the preferred molecular marker for detection of micro metastases in lymph nodes in almost all carcinomas. MATERIALS AND METHODS: Samples were collected from randomly chosen breast cancer patients undergoing modified mastectomy or breast conserving surgery between September 2003 and July 2004. RT-PCR was applied to study the expression of MMG and CEA markers. Breast cancer micrometastases in axillary lymph nodes were also assessed. RESULTS: The MMG marker was positive in 9/10 normal breast tissues, 3/3 breast fibroadenomas and 37/39 of breast carcinoma tissues, giving an overall sensitivity of 94%. The sensitivity was 80% for metastatic lymph node samples. On the other hand CEA showed 95% sensitivity for malignant breast tumors and 100% sensitivity for metastatic lymph nodes. CONCLUSIONS: RT-PCR using a combination of MMG and CEA markers is a powerful tool to complement current routine histopathology techniques for detection of breast cancer metastasis in axillary nodes.
Assuntos
Axila , Neoplasias da Mama/genética , Antígeno Carcinoembrionário/genética , Diagnóstico Precoce , Feminino , Fibroadenoma/genética , Humanos , Linfonodos/patologia , Metástase Linfática , Mastectomia , Proteínas de Neoplasias/genética , RNA Mensageiro/genética , RNA Neoplásico/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sensibilidade e Especificidade , Biomarcadores Tumorais/genética , Uteroglobina/genéticaRESUMO
The Iranian population includes several ethnic groups scattered throughout Iran, which differ with regard to their rates for cancer. An infamous 'Asian esophageal cancer belt'stretches to the east from the Caspian littoral in Iran via Turkmenistan to the Northern provinces of China. Apart from the eastern part of the Caspian littoral area of Iran which has the highest incidence of esophageal cancer in the world, other parts of the country have variable rates, ranging from 3 to more than 15 cases per 100,000 population, but rates have decreased sharply in the high incidence areas. Studies of the etiology of esophageal cancer in Iran and especially among the Turkmen ethnic population have indicated that several factors are behind the striking high incidence. Certain risk factors such as a family history of esophageal cancer, low socioeconomic status and poor nutritional diet are well studied and plausible evidence for their etiologic contributions exist. While thermal irritation and coarse food (physical damage to the mucosal lining of the esophagus) have attracted attention in correlational studies, conclusions about their etiologic contribution are inconclusive.