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Aim: In recent years, natural products from medicinal plants have received considerable attention. Thymoquinone, the major compound from Nigella sativa L. Is the most used due to its several pharmacological properties. This study was undertaken to evaluate the antioxidant effect of thymoquinone which is very useful in controlling free radicals noxious species causing the induction and / or amplification of a number of pathologies. Study Design: Spectrophotometric methods. Place and Duration of Study: Biochemistry Department, Applied Biochemistry Laboratory, Nature and Life Sciences Faculty, Ferhat Abbas Setif 1 University, Setif, Algeria, between December 2016 and September 2017. Methodology: In vitro antioxidant study was characterized by using free radicals scavenging methods with reducing power, lipid peroxidation and ß-carotene bleaching assays. All tests were realized by spectrophotometric methods. Results: Our study showed that thymoquinone is a potent antioxidant. It is less effective as a scavenger of both DPPH and ABTS radicals with IC50 of 125.65 ± 0.76 and 332.5 ± 14.39 µg/ml, respectively; While it is a strong scavenger of hydroxyl radical with IC50 of 26.3 ± 0.59 µg/ml and very strong hydrogen peroxide scavenger with IC50 of 11.0 ± 0.57 µg/ml. As superoxide anion scavenger it has inhibition ability less than 50%. In lipid peroxidation, TQ had a very efficient activity it inhibits peroxidation of β- carotene by 73.58 ± 0.50%. Thus, in lipid peroxidation assay, TQ had also a significant activity with percent inhibition of 79.5 ± 2.12%. Conclusion: Our results revealed that thymoquinone possesses a low antioxidant activity against DPPH, ABTS and superoxide anion radicals. Thus, it has a very low reducing power capacity, whereas it is a strong hydrogen peroxide, hydroxyl scavenger and lipid peroxidation inhibitor.
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Background: Natural products from medicinal plants such as thymoquinone (TQ), the major compound derived from Nigella sativa L. Which has received considerable attention in recent years due to its diverse pharmacological properties, including antioxidant and anti inflammatory activities? The aim of this study is the investigation of prophylactic and curative effects of TQ against carbon tetrachloride (CCl4)-induced hepatotoxicity in male albino rats. Materials and Methods: Hepatotoxicity was induced in rats by intraperitoneal administration of 3 ml/kg, 1:1 (V/V) mixture of CCl4 and olive oil both before and after treatment for 7 days with TQ. Prophylactic and curative effects of TQ were evaluated by estimating the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP). Results: CCl4 administering by intraperitoneal injection led to significant (P<0.05) increase in serum transaminases (ALT and AST) and phosphatase (ALP) respectively compared with control animals. Thymoquinone showed significant (p<0.05) hepatoprotective activity by decreasing the activities of ALT, AST, ALP in both prophylactic and curative effects. These results revealed that thymoquinone possesses significant hepatoprotective and hepatocurative effects against CCl4-induced toxicity via its antioxidant and anti-inflammatory activities but the hepatocurative effect was the most effective. Conclusion: Thymoquinone is a compound known for its pharmacological proprieties. We illustrated in this work, its important effect against hepatotoxicity induced by a toxic agent as CCL4 when it’s administrated in prophylactic or curative way.
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Objectif: Evaluer les resultats preliminaires de la mise en place d'une bandelette sous uretrale pour le traitement d'incontinence urinaire suite a une chirurgie prostatique chez l'homme selon la technique de Comiter modifiee (sans vissage osseux). Patients et methodes: Entre janvier 2005 et decembre 2006; 16 patients ont ete pris en charge pour incontinence urinaire qui apparut apres chirurgie prostatique. Une bandelette de polypropylene fixee sur le perioste des branches ischio-pubiennes par des fils de nylon et comprimant l'uretre bulbaire a ete mise en place. L'evaluation pre et post-operatoire a ete basee sur l'examen clinique et le nombre de protections utilisees par jour. Les patients ont ete revus a un mois post-operatoire; puis tous les trois mois. Resultats: Avec un recul moyen de 18 mois; 11 patients sont continents ne necessitant pas de protection et 3 patients sont nettement ameliores. Un echec a ete note chez un patient qui avait presente initialement une incontinence severe. Un autre patient a developpe une infection de la bandelette necessitant son retrait et a ete exclus de la serie pour l'evaluation fonctionnelle. Nous n'avons pas constate de douleurs perineales persistantes ni d'erosion uretrale ni d'osteite. Conclusion: Notre modification de la technique selon Comiter semble avoir des resultats satisfaisants comparables avec la technique originale. Elle presente un avantage economique certain par rapport a d'autres kits du marche