Cytokines [interferon gamma and interleukin-10] in oocyte and embryo culture media during in-vitro fertilization

The interleukin [IL] system has been shown to be intimately involved in embryonic implantation. The aim of this study is to elucidate whether human oocyte/embryo secrete cytokines like IFN- gamma and IL- 10 and effect of the balance between this cytokines on the fertilization process. A total of 207 oocyte/embryo culture media from 22 patients were collected and the cytokines level were tested by ELISA. The present study showed that IFN- gamma and IL- 10 were detected in 40.1% and 30.4% of oocyte/embryo culture media respectivity. There is significant difference between IFN- gamma and IL-10 levels in the media from fertilized oocytes between day 1 and day 2 [0.48 vs 1.49 and 34.2 vs 13.8] respectively. On the other hand, there was no significant difference between the IFN- gamma level of the media from fertilized and non fertilized oocytes 0.48 vs 0.85 at day 1 and 1.49 vs 1.49% at day 2 as well as IL-10 level 34.2 vs 30.9 at day 1 and 13.8 vs 9.48 at day 2 respectivily with conclusion that human oocytes and embryos secrete cytokines INF- gamma and IL-10 with variable concentration with no effect of these cytokines on fertilization process

Automatic fetal movement detection [fectal actogram] in cases of high risk pregnancy

This study was designed to compare automatic fetal movement detection [Fetal Actogram] with daily fetal movement count [DFMC] versus the st and ard real time ultrasound [RT/US]. 48 high risk pregnant women. admitted in Obstetrics and Gynecology Department of Tanta University Hospitals, from April 1997 to December 1997 were enrolled. Fetal movements [FMs] were evaluated by daily fetal movement count [DFMC], by automatic fetal movement detection [Fetal Actogram] and by RT/US Acceptable FMs were detected clinically, by actogram and by RT/US in 60.4%, 72.7% and in 81.25% of cases. The sensitivity, specificity and accuracy of Actogram for detection of acceptable FMs, they were 89.7%, 100%% and 91.7% respectively. Those for alarming FMs were 100%, 97.8% and 97.9% respectively. Fetal actogram identified 548 total FMs out of total 641 FMs by RT/US [85.4%]. It identifies 88.8% of complex FMs. 94.2% of isolated trunk movements and 93.7% of limb movements fetal Actogram evaluates FMs without extra testing if done with NST It gives good results in quantitative and qualitative evaluation of FMs