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1.
Mem. Inst. Oswaldo Cruz ; 102(6): 713-717, Sept. 2007. ilus, graf
Artigo em Inglês | LILACS | ID: lil-463477

RESUMO

The aim of this study was to test if serological distinction between patients with active and inactive neurocysticercosis (NCC), could be accomplished by the recognition of immunodominant peptides in total saline antigenic extract of Taenia solium metacestodes by IgG antibody in cerebrospinal fluid (CSF) and serum paired samples. CSF and serum samples of 10 each, active NCC patients, inactive NCC, and individuals with other neurological disorders, were used to recognize the antigenic peptides by western blot (WB). In the active NCC the 28-32 and 39-42 kDa peptides were more frequently detected in CSF than in sera (p < 0.05). The 47-52, 64-68, and 70 kDa antigens showed high frequencies in both samples from patients with active NCC. All the CSF samples of inactive NCC and other neurological disorder (control) patients tested negative, while serum samples from these last two groups recognized mainly the 80, 86, 95, and 98 kDa bands. This finding eliminates the use of the high molecular weigh bands (> 80 kDa) for diagnosis of NCC. The final conclusions were that the difference between active and inactive NCC may be done with the detection of peptides only in the CSF samples and that the 47-52, 64-68, and 70 kDa bands may be included as specific markers for active NCC when detected in CSF samples by WB using total saline extract of T. solium metacestode.


Assuntos
Animais , Humanos , Imunoglobulina G/imunologia , Neurocisticercose/diagnóstico , Peptídeos/imunologia , Taenia solium/imunologia , Anticorpos Anti-Helmínticos/sangue , Anticorpos Anti-Helmínticos/líquido cefalorraquidiano , Western Blotting , Imunoglobulina G/sangue , Imunoglobulina G/líquido cefalorraquidiano , Sensibilidade e Especificidade
2.
Mem. Inst. Oswaldo Cruz ; 100(4): 427-429, July 2005. tab, graf
Artigo em Inglês | LILACS | ID: lil-405999

RESUMO

Paired samples of cerebrospinal fluid (CSF) and serum of 30 patients - 10 with active, 10 with inactive neurocysticercosis (NCC), and 10 control subjects - were evaluated by enzyme-linked immunosorbent assay (ELISA) using two Taenia crassiceps metacestode extracts as antigen in order to detect IgG antibodies. In active NCC, high levels of IgG were detected (p < 0.05). The CSF samples showed 80 percent (CI 72-88) of reactivity in the saline extract (S) and 90 percent (CI 84-95) in sodium dodecyl sulphate (SDS) and the serum samples were reactive in 90 percent (CI 84-95) and 100 percent (CI 98-100) in the S and SDS antigenic extracts, respectively. The use of the paired samples of CSF and serum in active NCC showed equivalent results suggesting that the serum samples could be used as a screening in those patients whose CSF puncture is counter-indicated.


Assuntos
Humanos , Animais , Masculino , Feminino , Anticorpos Anti-Helmínticos/sangue , Anticorpos Anti-Helmínticos/líquido cefalorraquidiano , Antígenos de Helmintos , Imunoglobulina G/sangue , Imunoglobulina G/líquido cefalorraquidiano , Neurocisticercose/diagnóstico , Estudos de Casos e Controles , Reações Cruzadas , Ensaio de Imunoadsorção Enzimática , Taenia solium/imunologia
3.
Arq. neuropsiquiatr ; 52(1): 82-6, mar. 1994. ilus
Artigo em Português | LILACS | ID: lil-129371

RESUMO

O envolvimento do sistema nervoso central na paracoccidioidomicose tem sido raramente descrito na literatura e sua frequência varia de 9,99 por cento a 27,27 por cento, manifestando-se basicamente sob duas formas clínicas: meningea e pseudotumoral (abscesso, granulomas, nódulos ou cistos). O Paracoccidioides brasiliensis incide principalmente nos hemisférios cerebrais, podendo acometer ainda cerebelo, ponte, bulbo, meninges cerebrais e raquidianas, sendo excepcional o comprometimento do parênquima medular. Os autores apresentam o caso de um paciente com paracoccidioidomicose com evidências clínicas de comprometimento medular, comprovada por exames complementares indiretos pouco invasivos. Destacam a resposta terapêutica inédita a novo agente antifúngico bistriazólico, fluconazol, pela primeira vez utilizado nesta forma de apresentaçäo clínica da doença. Salientam a rariedade do comprometimento medular, o diagnóstico através de propedêutica cirúrgica e a excelente resposta a este novo tratamento


Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Fluconazol/administração & dosagem , Mielite Transversa/etiologia , Paracoccidioidomicose/complicações , Imageamento por Ressonância Magnética , Mielite Transversa/diagnóstico , Mielite Transversa/tratamento farmacológico , Paracoccidioides/isolamento & purificação , Paracoccidioidomicose/diagnóstico , Paracoccidioidomicose/tratamento farmacológico , Medula Espinal/patologia
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