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1.
Mem. Inst. Oswaldo Cruz ; 97(8): 1101-1105, Dec. 15, 2002. tab, graf
Artigo em Inglês | LILACS | ID: lil-326320

RESUMO

Clone CL B5 of Trypanosoma cruzi is a beta-galactosidase expressing organism that was genetically transfected to be used for in vitro pharmacological screening. Biological parameters were determined, evaluating growth kinetics of epimastigotes, metacyclogenesis, infectivity to mammalian cell lines, parasitemia kinetics in mice and sensibility to nifurtimox and benznidazole. Differences in relation to other strains and CL parental strain were found, the most important being the incapability to produce death to mice in spite of the high inoculum used. However, it possesses the required features to be used for in vitro drug screening. Data obtained demonstrate that heterogeneity of T. cruzi appears even among clones of the same strain, and that these differences found do not prevent the use of clone CL B5 for the purpose that was engineered


Assuntos
Animais , Camundongos , beta-Galactosidase , Trypanosoma cruzi , beta-Galactosidase , Clonagem de Organismos , Nifurtimox , Nitroimidazóis , Parasitemia , Testes de Sensibilidade Parasitária , Tripanossomicidas , Trypanosoma cruzi
2.
Mem. Inst. Oswaldo Cruz ; 97(4): 553-557, June 2002. ilus, graf
Artigo em Inglês | LILACS | ID: lil-314529

RESUMO

Nitroarylidenemalononitriles and their cyanoacetamide derivatives with remarkable anti-epimastigote properties, were synthesized attempting to obtain new 3,5-diamino-4-(5'-nitroarylidene)-4H-thiadiazine 1,1-dioxide derivatives, which in previous reports had shown anti-Trypanosoma cruzi activity. Tests to evaluate the cytotoxicity of compounds were performed on J774 macrophages. 5-nitro-2-thienyl-malononitrile (5NO2TM), was the only product which maintained a high anti-epimastigote activity at concentrations in which it was no longer cytotoxic, thus it was assayed against intracellular amastigotes. Its anti-amastigote activity was similar to that of nifurtimox. Afterwards in vivo toxicity and anti-chagasic activity were determined. A reduction in parasitemia was observed


Assuntos
Animais , Camundongos , Macrófagos , Nifurtimox , Tripanossomicidas , Trypanosoma cruzi , Nifurtimox , Testes de Sensibilidade Parasitária , Tripanossomicidas
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