Effects of Codonopsis pilosula water extract on MicroRNA expression profile in D-galactose-induced senile mice

This paper aims to observe and analyze effects of Codonopsis pilosula water extract on micro RNA [miRNA] expression profile in liver tissue of senile mice. The 110 Konminmice were randomly divided into five groups, including D-galactose-induced senile model group, normal control group, and low, middle and high dose intervention groups. Continuous modeling lasted 40 days. General symptoms and changes of body mass of the model mice were monitored and observed. The levels of serum glutamic pyruvic transaminase [ALT] and alkaline phosphatase [ALP] of mice were compared, and miRNA of differential expression during aging of D-galactose-induction and high-dose Codonopsis pilosula intervention was analyzed. The serum ALT and ALP levels in the aging model group were significantly higher than those in the normal control group [P<0.05]. The serum ALT and ALP levels of Codonopsis pilosula intervention group were lower than those of aging model group, and decrease in ALP value of high dose intervention group was higher [P<0.05]. The expression profile of miRNA in the aging model group was significantly different from that in normal control group and high-dose Codonopsis pilosula intervention group, and miRNA expression profile in high-dose Codonopsis pilosula intervention group was clustered with that in the normal control group. The differentially expressed miRNAs of D-galactose-induced senescence and Codonopsis pilosula anti-aging usually belong to 7 miRNA clusters. The target gene function of the differentially expressed miRNAs during senescence process was enriched in 29 signal pathways. There were 67 regulatory signal pathways in differentially expressed miRNA target genes during Codonopsis pilosula intervention. The effect of miRNA targeting may play an important role during D-galactose-induced senescence and Codonopsis pilosula anti-aging period

Paired observation on light-cured composite resin and nano-composite resin in dental caries repair

To compare the value of light-cured composite resin with that of nano-composite resin in dental caries repair. 88 patients taking dental caries repair in our hospital from May 2014 to April 2015 were selected, and divided into observation group and control group by coin method with 44 patients in each group. Nano-composite resin was used in observation group, while light-cured composite resin in control group. Then, the occurrence rates of odontohyperesthesia, aesthetic satisfaction with dental caries repair and complications were compared between two groups by visual analogue scale [VAS]. The occurrence rate of odontohyperesthesia in observation group is significantly lower than that in control group [9.09% [4/44] vs 31.82% [14/44], 6.82% [3/44] vs 22.73% [10/44], 2.27% [1/44] vs 13.64% [6/44]] with difference of statistical significance [P<0.05] 1 week, 4 weeks, and 8 weeks after repair. VAS score of patients in observation is significantly lower than that in control group [[2.78 +/- 0.56] scores vs [5.22 +/- 0.76] scores, [2.02 +/- 0.35] scores vs [4.32 +/- 0.57] scores, [1.12 +/- 0.14] scores vs [2.41 +/- 0.43] scores] 1 week, 4 weeks, and 8 weeks after repair. Moreover, the difference in comparison of interactive effects between two groups, between different time points, and between groups at different time points has statistical significance [P<0.05]. Nano-composite resin can lower the occurrence rate of odontohyperesthesia in dental caries repair, reduce the pain of patients, and improve the satisfaction of patients with tooth appearance