Effect of Galectin-3 Gene Transfer for the Apoptosis and Cell Growth of the Prostate Cancer Cell Line (LNCaP Cells) / 대한비뇨기과학회지

PURPOSE: Galectin-3 is a member of a large family of beta-galactoside- binding animal lectins. It is thought that galectin-3 can be a suppressor of apoptosis because of its significant sequence similarity with Bcl-2. We examined the role of galectin-3 for the paclitaxel-induced apoptosis after transfection of the galectin-3 gene in LNCaP cells. MATERIALS AND METHODS: Galectin-3 cDNA was cloned into PcDNA 3.1(-) and transfected into LNCaP cells. Stable transfection of galectin-3 into the LNCaP cells was achieved. Growth of the transfectants was observed with performing MTT assay. Apoptosis was induced by 100nM paclitaxel and 2microM staurosporine, and this was observed by DNA fragmentation assay. The viable cell numbers(% of control) after induction of apoptosis were determined with performing MTT assay. RESULTS: The LNCaP subclone that expressed galectin-3(LNCaP-G3-PcDNA) grew faster than the control transfectant(LNCaP-PcDNA)(p<0.05). The DNA fragmentation bands were decreased in the LNCaP subclone expressing galectin-3(LNCaP-G3-PcDNA) as compared to the control transfectant(LNCaP-PcDNA) after induction of apoptosis by 100nM paclitaxel or 2iM staurosporine; this means that galectin-3 inhibits apoptosis. The viable cells (% of control) with LNCaP-G3-PcDNA after the induction of apoptosis by 100nM paclitaxel was 92+/-2% in 8 hours, 77.5+/-1.9% in 24 hours and 40.4+/-2.9% in 48 hours on average, respectively. In contrast, the viable cells(% of control) of the control transfectant were 84.5+/-2%, 46+/-2.5% and 19+/-2.6% on average, respectively. The viable cells(% of control) with the LNCaP-G3-PcDNA after the induction of apoptosis by 100nM paclitaxel was higher than that of the control transfectant(LNCaP- PcDNA cells)(p<0.05). CONCLUSIONS: Galectin-3 gene transfer stimulates the growth of LNCaP cells. The galectin-3 protects LNCaP cells from paclitaxel-induced apoptosis.

Two Case of Cutaneous Horn / 대한피부과학회지

Cutaneous horn is a clinical decription for a cohesive mass of cornified mterial protruding from the surface of the skin. The incidence of cutancous horn is relaively rare and the lesion is usually single, The predilection sites are known to be the exposed area of the skin, that is, face, ear, dorsum cf hand etc. Soles are reported as the least common site of the cutaneous horn. Here, we report two cases of cutaneous horn. The one was a 49 year old schzophrenic male who had multiple cone shaped protrusions on hoth soles for 10 year. The other was 17 year old, boy who had a single cornified protrusion on the left forhead. The histopa.thologic findings from the base of two cases were consistent with benign hyperplastic epithelium.