RESUMO
Infertility is a problem concerning 10-15% of the individuals in the fertile period. This study investigated effects of proinflammatory factors as well as lipid hydroperoxides [LPO] levels upon in vitro fertilization [IVF] success. In this prospective, non-randomized, controlled clinical study, sera obtained from 26 fertile [group-1], 26 infertile women before [group-2] and after [group-3] IVF treatment were analyzed. Leptin, leptin receptor, resistin, tumor necrosis factor-alpha [TNF-alpha], and C-reactive protein [CRP] were analyzed using enzyme-linked immunosorbent assay [ELISA]. LPO was determined spectrophotometrically. Mann-Whitney U test, paired samples t test, Wilcoxon signed-rank test as well as Pearson correlation analysis by SPSS were performed for statistical analysis. TNF-alpha, resistin and LPO levels increased [P=0.020, P=0.003, P=0.001, respectively] in group-3 compared to group-2. A significant increase in LPO was noted both in group-2 and -3 compared to controls [P=0.000]. LPO were higher in non-pregnants than pregnants in group-2. For pregnants, significant correlations were observed between leptin and resistin in group-2 and TNF-a and leptin in group-3. None of these correlations were found for the women, who could not conceive. LPO, leptin-resistin correlation, associations with TNF-a may be helpful during the interpretation of IVF success rates
RESUMO
Possible roles of anti-inflammatory factors as well as total antioxidative capacity in reproductive processes of women undergoing in vitro fertilization [IVF] are still being investigated and the contributions by some of them remain controversial. The aim of this study is to investigate the relationship between anti-inflammatory parameters and total antioxidative capacity [TAC] of the body during IVF. In this respect, adiponectin, interleukin-10 [IL-10], interleukin-1 receptor antagonist [IL-1RA], fetuin A and TAC analyses have been performed. In this prospective, non-randomized, controlled clinical study, sera obtained from 26 fertile [Group-1], and 26 infertile women before [Group-2] and after [Group-3] IVF treatment were analyzed. IL-1RA, IL-10, fetuin A, adiponectin and insulin were determined by ELISA. TAC was determined spectrophotometrically. Mann-Whitney U test, paired sample t-test, Wilcoxon signed-rank test as well as Pearson correlation analysis by SPSS were performed for statistical analysis. Clinical pregnancy and live birth rates were determined as 30.8% and 23.1%, respectively, in pregnant group. For the pregnant, significant indirect correlations were detected between fetuin A and adiponectin [r=-0.843; p=0.035] as well as IL-10 [r=-0.846; p=0.034] in Group 2. The correlation between adiponectin and IL-10 doubled in pregnant compared to non-pregnant [r=0.929; p=0.007 vs. r=0.478; p=0.033]. The correlations between fetuin A and TAC in pregnant were noted both in Group 2 [r=0.892; p=0.017] and Group 3 [r=0.875; p=0.022]. No correlation of fetuin A with these parameters was detected in non-pregnant group. Fetuin A, TAC, IL-10, adiponectin and their associations may be important from their predictive values for IVF success point of view. Parameters with anti-inflammatory or antioxidant property appear to improve pregnancy in women undergoing IVF