Perception and prejudice about organ donation among medical students in a tertiary healthcare, Mysuru

Background: Today, increase in deaths due to organ failure can be avoided if organ donors are available. Many states in India adopted the transplantation of human organs act and enthusiastic medical professionals and philanthropists joined hands to move the matter further forward. Lack of awareness about organ donation has been noted among the general public including medical professionals. Awareness can be promoted through medical students to bridge the gap of knowledge about organ donation and they can carry the message to the community. Hence an attempt has been made to probe this issue with objective to assess the perception and prejudice about organ donation among medical students.Methods: This cross-sectional study was conducted among medical students in a tertiary healthcare centre, Mysuru. A total of 201 students from 1st and 3rd term, consenting to participate were included in the study. A semi-structured self-administered questionnaire was used to collect data and was analysed using SPSS 22 version.Results: Knowledge about brain death was 73.1%, transplant of harvested organs was 59%, compatibility tests were 89% and organs donated by live donors were 89.6% for kidneys and 66.7% for cornea in cadaver donors. Regarding legal issues 73% knew that organ selling is criminal offence. 90% supported organ donation and related to prejudice 53% agreed that there are no religious constraints to donate organs.Conclusions: Knowledge and awareness regarding procedure, pre-requisites and legal issues related to organ donation needs to be improved among the medical students.

Prevalence and severity of depression among people residing in flood affected areas of Kerala

Background: The world is witnessing significant change in its climate leading to an increase in natural disasters. Kerala state of India recently witnessed its worst Monsoon rainfall in the century. Floods have significant effects on mental health. Screening of psychological problems is necessary in the background of recent floods in Kerala. So this study was done with the objectives to assess the prevalence of depression in the flood affected people of Kerala and to assess the severity of depression among people residing in flood affected areas of Kerala.Methods: Cross sectional study was done four weeks after flood. Individuals aged ≥15 years residing in flood affected areas were included and people not willing to participate were excluded. Purposive sampling was done and 306 participants were studied. Participants were assessed by interview technique using PHQ9 questionnaire. Data was analysed using SPSS-22. Descriptive statistics like percentage were applied. Inferential statistical tests like chi-square test were applied for association.Results: Among the participants 48% showed symptoms of depression. 28.10 % were mildly depressed, 12.74% were moderately depressed, 5.56% had moderately severe and 1.63% had severe depression. Maximum prevalence of depression was observed in geriatric age group.Conclusions: A significant amount of people residing in flood affected areas showed signs and symptoms of depression. An urgent intervention is therefore required to improve mental health status of the flood affected individuals.

Public health concern on occupational hazards among pathologists and microbiologists in Mysuru district, India

Background: Highly qualified and trained medical specialists such as Pathologists and Microbiologists who need to work at the microscope for long hours are at a high risk of musculoskeletal and ophthalmic disorders. This study is an attempt to identify such potential occupational hazards with the objectives to estimate the magnitude of occupational hazards among Pathologists and Microbiologists in Mysuru, and to assess the factors influencing these hazards among the study participants.Methods: This cross-sectional study was conducted among the Pathologists and Microbiologists of Mysuru district over a period of six months, a total of 45 study participants were included. An online self-administered questionnaire was sent to the study participants through e-mail and asked to fill and submit online, keeping complete confidentiality, and with informed consent. The results were analysed using appropriate statistical methods.Results: Pathologists and Microbiologists in almost equal proportions consented and took part in the study. 67% of them reported work related musculoskeletal problems, and 42% had visual refractive errors, mostly myopia. 9% of them reported to have experienced occupational injuries, mostly needle-stick injuries. None of them reported to smoke. Majority were happy with the work atmosphere and environmental conditions. Most of them were positive about the work-related situation in the coming years.Conclusions: This study found that more than half of the pathologists and microbiologists are exposed to occupational hazards and have suffered musculoskeletal, ophthalmic morbidities and had injuries, but none of which were life-threatening. Most of the risk factors are avoidable through ergonomic equipment and training.

Patient based factors influencing drug compliance among hypertensive patients in a tertiary care hospital in Mysore.

Background: Hypertension is deemed as the tip of the iceberg due to the mortality and morbidity associated with it. A major factor accounting for inadequate treatment of hypertension is poor compliance. Methods: Morisky 8-Item Medication Adherence Questionnaire was used for a cross sectional study. The term compliance is defined as the extent to which the patient’s behaviour coincides with the clinical prescription, implying that the patient defaults by not following the advice of the health care provider Results: Mean age of the participants was 59.2yrs (S.D. 10.37 yrs). Compliance was found to be good 71.3% of respondents, medium in 20.4% and poor in 8.3%.52.8% had one or two other ailments (diabetes, asthma etc.). Conclusions: Patient’s medication compliance is a multifactor behaviour in which the role of patient’s attitude is very important. Patients related factors known to affect compliance were equally distributed among good, medium and poorly compliant participants

Structure and function of enzymes involved in the anaerobic degradation of L-threonine to propionate.

In Escherichia coli and Salmonella typhimurium, L-threonine is cleaved non-oxidatively to propionate via 2-ketobutyrate by biodegradative threonine deaminase, 2-ketobutyrate formate-lyase (or pyruvate formate-lyase), phosphotransacetylase and propionate kinase. In the anaerobic condition, L-threonine is converted to the energy-rich keto acid and this is subsequently catabolised to produce ATP via substrate-level phosphorylation, providing a source of energy to the cells. Most of the enzymes involved in the degradation of L-threonine to propionate are encoded by the anaerobically regulated tdc operon. In the recent past, extensive structural and biochemical studies have been carried out on these enzymes by various groups. Besides detailed structural and functional insights, these studies have also shown the similarities and differences between the other related enzymes present in the metabolic network. In this paper, we review the structural and biochemical studies carried out on these enzymes.

Source and target enzyme signature in serine protease inhibitor active site sequences.

Amino acid sequences of proteinaceous proteinase inhibitors have been extensively analysed for deriving information regarding the molecular evolution and functional relationship of these proteins. These sequences have been grouped into several well defined families. It was found that the phylogeny constructed with the sequences corresponding to the exposed loop responsible for inhibition has several branches that resemble those obtained from comparisons using the entire sequence. The major branches of the unrooted tree corresponded to the families to which the inhibitors belonged. Further branching is related to the enzyme specificity of the inhibitor. Examination of the active site loop sequences of trypsin inhibitors revealed that here are strong preferences for specific amino acids at different positions of the loop. These preferences are inhibitor class specific. Inhibitors active against more than one enzyme occur within a class and confirm to class specific sequence in their loops. Hence, only a few positions in the loop seem to determine the specificity. The ability to inhibit the same enzyme by inhibitors that belong to different classes appears to be a result of convergent evolution.

Studies on simultaneous inhibition of trypsin and chymotrypsin by horsegram Bowman-Birk inhibitor.

Bowman-Birk inhibitors (BBI) isolated from plant seeds are small proteins active against trypsin and/or chymotrypsin. These inhibitors have been extensively studied in terms of their structure, interactions, function and evolution. Examination of the known three-dimensional structures of BBIs revealed similarities and subtle differences. The hydrophobic core, deduced from surface accessibility and hydrophobicity plots, corresponding to the two tandem structural domains of the double headed BBI are related by an almost exact two-fold, in contrast to the reactive site loops which depart appreciably from the two-fold symmetry. Also, the orientations of inhibitory loops in soybean and peanut inhibitors were different with respect to the rigid core. Based on the structure of Adzuki bean BBI-trypsin complex, models of trypsin and chymotryspin bound to the monomeric soybean BBI (SBI) were constructed. There were minor short contacts between the two enzymes bound to the inhibitor suggesting near independence of binding. Binding studies revealed that the inhibition of one enzyme in the presence of the other is associated with a minor negative cooperativity. In order to assess the functional significance of the reported oligomeric forms of BBI, binding of proteases to the crystallographic and non-crystallographic dimers as found in the crystal structure of peanut inhibitor were examined. It was found that all the active sites in these oligomers cannot simultaneously participate in inhibition.

Primary structure of sesbania mosaic virus coat protein: its implications to the assembly and architecture of the virus.

Sesbania mosaic virus (SMV) is a plant virus that infects Sesbania grandiflora plants in Andhra Pradesh, India. The amino acid sequence of the coat protein of SMV was determined using purified peptides generated by cleavage with trypsin, chymotrypsin, V8 protease and clostripain. The 230 residues so far determined were compared to the corresponding residues of southern bean mosaic virus (SBMV), the type member of sobemoviruses. The overall identity between the sequences is 61.7%. The amino terminal 64 residues, which constitute an independent domain (R-domain) known to interact with RNA, are conserved to a lower extent (52.5%). Comparison of the positively charged residues in this domain suggests that the RNA-protein interactions are considerably weaker in SMV. The residues that constitute the major domain of the coat protein, the surface domain (S-domain, residues 65-260), are better conserved (66.5%). The positively charged residues of this domain that face the nucleic acid are well conserved. The longest conserved stretch of residues (131- 142) corresponds to the loop involved in intersubunit interactions between subunits related by the quasi 3-fold symmetry. A unique cation binding site located on the quasi 3-fold axis contributes to the stability of SMV. These differences are reflected in the increased stability of the SMV coat protein and its ability to be reconstituted with RNA at pH 7.5. A major epitope was identified using monoclonal antibodies to SMV in the segment 201-223 which contains an exposed helix in the capsid structure.(ABSTRACT TRUNCATED AT 250 WORDS)

Crystal structure of cadaverine dihydrochloride monohydrate.

The structure of cadaverine dihydrochloride monohydrate has been determined by X-ray crystallography with the following features: NH3+ (CH2)5NH3+.2Cl-.H2O, formula weight 191.1, monoclinic, P2, a = 11.814(2)A, b = 4.517(2)A, c = 20.370(3)A, beta = 106.56 degrees (1): V = 1041.9(2)A3; lambda = 1.541A; mu = 53.41; T = 296 degrees; Z = 4, Dx = 1.218 g.cm-3, R = 0.101 for 1383 observed reflections. The crystal is highly pseudo-symmetric with 2 molecules of cadaverine, 4 chloride ions and 2 partially disordered water molecules present in the asymmetric unit. Though both the cadaverine molecules in the asymmetric unit have an all trans conformation, the carbon backbones are slightly bent. Between the concave surfaces of two bent cadaverine molecules exists water channels all along the short b axis. The water molecules present in the channels are partially disordered.

Crystal and molecular structure of sym-homospermidine monohydrate.

Sym-homospermidine, [formula; see text] is a naturally occurring rare-polyamine found in relatively large concentration in sandal leaves. As part of our studies on structure and interactions of polyamines, sym-homospermidine was purified from sandal leaves and its structure was determined by single crystal X-ray diffraction technique. The phosphate salt of the molecule crystallized in the triclinic space group P1- with a = 8.246(1)A, b = 8.775(1)A, c = 15.531(2)A, alpha = 74.20(1) degrees, beta = 88.36(1) degrees and gamma = 65.41(1) degrees. The structure was determined by direct methods and refined to a final R factor of 5.4% for 2087 reflections with magnitude of F(obs) greater than 5 sigma [F(obs)]. The amine exists in its most favourable all trans conformation. For each amine molecule three phosphate groups exist in the crystal structure, suggesting that two of the oxygens of each phosphate group are protonated. There is also a single water molecule in the asymmetric unit in contrast to that of spermidine phosphate which has 3 water molecules. These differences probably reflect the hydrogen bonding properties of mono-ionic and di-ionic phosphate groups. The structure is predominantly stabilized by a network of hydrogen bonds.

Similarities in the genomic sequence and coat protein structure of plant virsuses.

The genomic sequences of several RNA plant viruses including cucumber mosaic virus, brome mosaic virus, alfalfa mosaic virus and tobacco mosaic virus have become available recently. The former two viruses are icosahedral while the latter two are bullet and rod shaped, respectively in particle morphology. The non-structural 3a proteins of cucumber mosaic virus and brome mosaic virus have an amino acid sequence homology of 35% and hence are evolutionarily related. In contrast, the coat proteins exhibit little homology, although the circular dichroism spectrum of these viruses are similar. The non-coding regions of the genome also exhibit variable but extensive homology. Comparison of the brome mosaic virus and alfalfa mosaic virus sequences reveals that they are probably related although with a much larger evolutionary distance. The polypeptide folds of the coat protein of three biologically distinct isometric plant viruses, tomato bushy stunt virus, southern bean mosaic virus and satellite tobacco necrosis virus have been shown to display a striking resemblance. All of them consist of a topologically similar 8-standard β-barrel. The implications of these studies to the understanding of the evolution of plant viruses will be discussed.

Evolutionary relationship of alfalfa mosaic virus with cucumber mosaic virus and brome mosaic virus.

The amino acid sequences of the non-structural protein (molecular weight 35,000; 3a protein) from three plant viruses – cucumber mosaic, brome mosaic and alfalfa mosaic have been systematically compared using the partial genomic sequences for these three viruses already available. The 3a protein of cucumber mosaic virus has an amino acid sequence homology of 33.7% with the corresponding protein of brome mosaic virus. A similar protein from alfalfa mosaic virus has a homology of 18.2% and 14.2% with the protein from brome mosaic virus and cucumber mosaic virus, respectively. These results suggest that the three plant viruses are evolutionarily related, although, the evolutionary distance between alfalfa mosaic virus and cucumber mosaic virus or brome mosaic virus is much larger than the corresponding distance between the latter two viruses.

Evolutionary relationship of alfalfa mosaic virus with cucumber mosaic virus and brome mosaic virus.

The amino acid sequences of the non-structural protein (molecular weight 35,000; 3a protein) from three plant viruses – cucumber mosaic, brome mosaic and alfalfa mosaic have been systematically compared using the partial genomic sequences for these three viruses already available. The 3a protein of cucumber mosaic virus has an amino acid sequence homology of 33.7% with the corresponding protein of brome mosaic virus. A similar protein from alfalfa mosaic virus has a homology of 18.2% and 14.2% with the protein from brome mosaic virus and cucumber mosaic virus, respectively. These results suggest that the three plant viruses are evolutionarily related, although, the evolutionary distance between alfalfa mosaic virus and cucumber mosaic virus or brome mosaic virus is much larger than the corresponding distance between the latter two viruses.