Effects suramin, tumor necrosis factor-alpha(TNF-alpha, interferon-alpha(IFN-alpha on human renal carcinoma cell line growth / 대한비뇨기과학회지

Recently much interest has been expressed for the use of biological response modifiers and growth factor antagonists in the treatment or radiation and chemotherapeutic drug-refractory renal cell carcinoma. Herein antiproliferative effects of Suramin, human recombinanl tumor necrosis factor alpha (TNF-alpha) and human recombinant interferon alpha (IFN-alpha) as a single and in combination were studied in vito on human renal carcinoma cell line (Caki-1). Antiproliferative effect was evaluated by trypan blue dye exclusion assay after 3 days exposure to Suramin at 10, 30, 100, 300, 1,000 mcg/ ml. TNF-alpha at 1, 10, 50, 100, 500, 1,000 units/ml and IFN-alpha at 10, 100, 1,000. 10,000 units/ml concentration, respectively. In addition, effects of combined administration in tolerable peak plasma level or suramin (300 mcg/ml). TNF-alpha (500 units/ml) and IFN-alpha (1000 units/ml) were comparatively analyzed to those of single drug administration. The results were as follows: 1. Significant dose dependent antiproliferative effects were shown by Suramin at above 100 mcg/ml. TNF-alpha at above 500 units/ml and IFN-alpha at above 10 units/ml, respectively (p<0.05). 2. At peak plasma level, suramin, TNF-alpha and IFN-alpha showed less than 50% inhibition of proliferation. 3. On combined administration, suramin plus TNF-alpha (61.0%), Suramin plus IFN-alpha(71.7%) and TNF-alpha plus IFN-alpha (57.2%) induced significantly greater inhibition of proliferation (p<0.005). These results suggest that further in vivo study using combination of Suramin plus TNF-alpha Suramin plus IFN-alpha and TNF-alpha plus IFN-alpha is necessary and that these combination trials may become one of the treatment options for renal cell carcinoma.

Evaluation of urinary enzyme levels after piezoelectric extracorporeal shock wave lithotripsy for renal stones / 대한비뇨기과학회지

Among the patients who underwent piezoelectric extracorporeal shock wave lithotripsy (ESWL) for renal stone between September l99l and December 1991, 20 patients non-selectively participated in this study. Of 20 patients 9 had renal pelvis stones and II had calyceal stones, and all received 45 average storage or shock waves. The changes of NAG, GOT. GPT, LDH, ALP, r-GTP in the blood and 24-hour urine were measured as a marker of renal damage before and after the first session of ESWL treatment. Serum levels of these enzymes did not appear to change with ESWL. As for the changes in the total volume and concentration of these enzymes in 24-hour urine. all 6 enzymes tended to increase though not significantly but the NAG significantly increased after ESWL. Therefore. these data suggest that the evaluation of urinary enzyme levels is an economic, rapid applicable diagnostic method after ESWL of renal stone. Especially. the urinary NAG assay is to be a brief screening test prior to radiographical study.