RESUMO
The extreme polymorphism in MHC B-F genes enables the chickens to recognize enormous numbers of foreign peptides to trigger an immune reaction. Study of these genetic resources is very important and it is essential to avoid the loss of genetic variability. Genetic polymorphism among Arian strain, a endogenous broiler chickens in Iran, was investigated. In this study PCR-SSCP was carried out for revising the polymorphism in MHC [B-F] in Arian broiler chicken. 65 samples of chicken DNA belonged to 2 groups of parents [25] and hybrids [40] were analyzed. 7 distinct profiles have been demonstrated for two groups. Two profiles were identical between two groups. Two of all profiles have been demonstrated by previous studies and 5 were new in this study. From this study it was concluded that MHC [B-F] diversity exists within Arian broiler chicken that might be useful for selecting desirable immunological traits
Assuntos
Animais , Genótipo , Galinhas , Reação em Cadeia da Polimerase , Polimorfismo Conformacional de Fita SimplesRESUMO
In this study one lymph node from cow with viral lymphosarcoma and one lymph node from healthy cow were analyzed by three different methods for protein extraction, include simple homogenizing in PBS, sonication and phenol extraction. Tumor antigen detection relies on immunoprecipitation followed by SDS-PAGE. In this experiment Streptococcus pyogenes was used for purification of immune complexes. Eletrophoretic patterns were obtained using reduced and non reduced buffers. In PBS homogenization 3 distinct antigens [39, 32 and 30 kDa] were observed. When sonication or phenol extraction were used, 5 [72, 48, 42, 32 and 30 kDa] and 6 [104, 77, 54, 32, 30 and 26, 5 kDa] distinct antigens were observed, respectively. This study showed that the ability of antigen detection mainly depends on protein extraction procedure and antigen-antibody equilibrium achieved by quantification of precipitins. Immunoprecipitation could be used successfully for study of tumor antigens in bovine leukosis