RESUMO
Introduction: is to determine the prevalence of the hemolysed samples and to study the interference of hemoloysis on the determination of 17 current biochemical parameters: 3 electrolytes [K[+], Ca[2][+], PO[4] [2]]; 8 substrates [cholesterol, triglycerides, total bilirubin, direct bilirubin, glucose, uric acid, rate of protids, creatinin], 6 enzymes [LDH, ASAT, GGT, CK, ALAT, ALP]
Material and method: The study, concerning 34 patients performed blood samples for each patient on dry tubes without freezing, on one of these 2 samples we carried out a hemolysis by agitation [mechanical manner], after centrifugation of the two tubes we made the biochemical parameters assay. The study of the prevalence of the hemolysis samples was carried out by counting the number of hemolysed samples among the whole of samples received at the laboratory during six weeks
Results: Potassium; cholesterol, tiglycerides, total bilirubin, direct bilirubin; uric acid, LDH, ASAT; ALAT, CK and ALP were positively influenced by hemolysis, whereas the influence was negative for glucose, and creatinin. calcium, phoshorus, rate of protids, and GGT did not show significant variations. The prevalence of the hemolysed samples in the clinical biochemistry laboratory was 4,8%
Conclusion: The most common biochemical parameters are influenced by hemolysis, which conducts us to insist on control of the pre-analytical phase of sampling