RESUMO
The purpose of this study was to investigate the effect of 6W power Carbon Dioxide Laser [CO[2]] on the biologic compatibility of the Sandblasting with large grit and acid etching [SLA] titanium discs through studying of the Sarcoma Osteogenic [SaOS-2] human osteoblast-like cells viability. Sterilized titanium discs were used together with SaOS-2 human osteoblast-like cells. 6 sterilized SLA titanium discs of the experimental group were exposed to irradiation by CO[2] laser with a power of 6W and 10.600nm wavelength, at fixed frequency of 80Hz during 45 seconds in both pulse and non-contact settings. SaOS-2 human osteoblast-like cells were incubated under 37 C in humid atmosphere [95% weather, 5% CO[2]] for 72 hours. MTT test was performed to measure the ratio level of cellular proliferation. The results indicated that at 570nm wavelength, the 6W CO[2] laser power have not affected the cellular viability. CO[2] laser in 6w power has had no effect on the biologic compatibility of the SLA titanium surface
RESUMO
Osteon has been introduced as a bone substitute material. It is biphasic calcium phosphate [BCP] that use in dentistry. The aim of the present study was to evaluate the effect of osteon on the proliferation, cell viability and differentiation of saos-2 cells in vitro. Also it was compared with cerasorb bone graft. Two different bone grafts materials, osteon and cerasorb, were used to evaluate the effect on proliferation and differentiation rate of saos-2 cells. On day 15 the cell proliferation and cell viability was measured by MTT assay. For determination of differentiation, alkaline phosphatase and alizarin red test was used. Osteon and cerasorb groups showed significantly higher alkaline phosphatase activity and cell differentiation. Cell viability of both bone grafts was significantly lower than control group and cell proliferation was higher in osteon group. Osteon has more suitable biological property compare to cerasorb. The results of the present study showed that osteon and cersorb bone grafts allow proliferation and differentiation of saos-2 cells in vitro
RESUMO
Several reports on clinical utility of various Bone Substitute Materials, [BSM] for grafting have shown successful results. The most important factor in successful grafting of BSM is the physiological and histological behavior of this material which can be evaluated by observing, rate of survival and proliferation of osteogenic cells. This research was conducted to examine the effect of Totudent and Bio-oss on the morphology, proliferation and rate of survival of Saos-2 osteoblastoid cells in vitro. This experimental study was done on two types of materials used as bone grafts viz. Totudent and Bio-oss. Twelve samples from each of these 2 materials grafted on Saos-2 osteoblastoid cells and 4 negative control samples were studied. On day 15, the rate of cell survival was determined by the MTT test and on day 20, Alkaline phosphatase activity of these cells in the culture medium were evaluated by the Alkaline phosphatase kit. The rate of cell survival in the Bio-oss and Tutodent group was significantly diminished in comparison with the control group. This rate was significantly higher in the cells grafted with Tutodent as compared to Bio-oss; alkaline phosphatase activity in Totudent group was higher than the other 2 groups. Tutodent bone graft showed better result in the morphologic variations of Saos-2 cells in the culture medium as compared to Bio-oss, and has more appropriate biologic characteristics