RESUMO
Tumeric is a perennial herb and member of Zingiberaceae [ ginger] family. The portion of the plant used as a yellow powder which is used as a flavor in many cuisines and as a traditional medicine to treat some diseases. In this study the cytotoxic activity of Turmeric against human liver tumor cell lines [HEPG2], colon carcinoma [HCT] and tumor breast carcinoma [MCF7] were evaluated. Total phenols contents [TPC] and fractionation of poly phenolic and flavonoids compounds in turmeric were determined. The scavenging effect of DPPH [2,2- diphenyl-1-picrylhydrazyl] radicals was evaluated. The organoleptic properties of wheat pie was tested with different levels [0%,5%,10% and 15%] of tumeric powder. The results showed that turmeric exhibited an efficient cytotoxicity against HEPG2, MCF7 and HCT carcinoma cell lines ; moreover turmeric extracts possess significant antioxidant properties and is rich in phenolic compound. Crust color in pie containing turmeric powder become darker, and it varied with increasing the quantity of turmeric powder. Substitution of wheat flour with 5% turmeric powder showed acceptable sensory scores comparable to wheat pie. Pie containing turmeric powder can be considered as a health-promoting functional food
RESUMO
The Antioxidant, anticancer and anti-inflammatory activities of black seed oil, sesame oil and olive oil were evaluated. The in vitro scavenging activities of the three oils on H[2]O[2] and DPPH radicals, microsomal lipid peroxidation, and the iron-chelating ability were studied. In addition, the anticancer activity; cytotoxicity of the three oils on liver [HEP G2], breast [MCF7] and colon [HCTI 16] human tumor cell lines were also tested using sulphorhodamine B [SRB] assay, DNA fragmentation by two methods: agarose gel electrophoresis and diphenylamine assays and two lysosomal enzymes: DNase II and cathepsin D. Caspase-3 activity was also determined. Furthermore, we have investigated alterations in the glutathione [GSH] and lipid peroxidation [LPO] level and the activities of antioxidative enzymes, catalase [CAT] and glutathione-s-transferase [GST] as markers of acute inflammation, following oral administration of the three oils and indomethacin in rats treated with carrageerian-induced paw oedema. The results showed that all of these oils by three concentrations: 25, 50 and 100 micro g ml[-1] scavenged H[2]O[2] and DPPH radicals and inhibited the lipid peroxidation process. The iron ions were chelated by the three oils. Moreover, the black seed oil and olive oil exhibited an efficient cytotoxicity against HEPG2, MCF7 and HCTI 16 carcinoma cell lines, while sesame oil did not maintain any cytotoxic property against all tumor cell lines under our experimental conditions. The cytotoxicity was confirmed by increase the activity of caspase-3 activity, DNase II and cathepsin D and DNA fragmentation. Also, the three oils at 50 and 100 mg kg[-1] orally produced significant anti-inflammatory effect after I hours of carrgeenan injection till 4 hours. The three oils alleviated increases in.the activities of CAT and GST enzymes and lipid peroxidation content resulting from oedema. Also they ameliorated depressions in the GSH level caused by carrageenan injection. Our results concluded that these oils can be used as a source of natural antioxidants and dietary supplements to prevent free radical diseases such as cancer, cirrhosis, diabetes and inflammation