RESUMO
Three yeast strains were used for production of microbial lipid globules using glucose as sole carbon source. These strains were: Candida tropicalis, C. utilis and C. Boidinii. The results showed that C. tropicalis was the most suitable organism for lipid production among the three different yeast strains under study. The efficient strain C. Tropicalis was grown on three different culture modes: Batch, twice fed batch and continuous for studying the most cultural mode for production of lipid. The lipid production rate during the second phase of twice fed batch culture was higher than that of batch culture using synthetic medium. The lipid production rate in continuous culture was higher than batch or twice fed batch, it was 2 - 3 times higher than the batch cultures. The obtained lipid was analyzed for their fatty acids composition. These compositions showed differences in their percentages of batch and continuous culture modes with C. Tropicals. Palmitic C 16: 0 and oleic C 18: 1 fatty acids were the dominant acids in both cultural modes. In batch culture C 16: 0 and C 18: 1 fatty acids were lower than those obtained from continuous culture, while unsaturated fatty acids C 18: 2 and C 18: 3 were higher